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ORIGINAL ARTICLE
Variation in the Phenolic Compounds Profile and Antioxidant Activity in Different Parts of Hawthorn (Crataegus pentagyna Willd.) During Harvest Periods
1
Department of Chemistry, Faculty of Science and Mathematics, University of Niš, Višegradska 33, 18000 Niš, Serbia
2
Department of Biochemistry, Faculty of Medicine, University of Niš, Bulevar dr Zorana Đinđića 81, 18 000 Niš, Serbia
Submission date: 2018-10-18
Final revision date: 2019-07-17
Acceptance date: 2019-08-29
Online publication date: 2019-11-07
Publication date: 2019-11-18
Corresponding author
Jovana Pavlovic
Department of Chemistry, Faculty of Science and Mathematics, University of Nis, Serbia
Department of Chemistry, Faculty of Science and Mathematics, University of Nis, Serbia
Pol. J. Food Nutr. Sci. 2019;69(4):367-378
KEYWORDS
TOPICS
ABSTRACT
Plants of the genus Crataegus, Rosaceae, are widely distributed and have long been used for food and in folk medicine for the treatment of various ailments. This study focused on HPLC-DAD-FLD identification of phenolic compounds of flowers, fruits, and leaves of hawthorn (Crataegus pentagyna Willd.), on monitoring changes in the content of phenolic compounds during different harvesting periods, as well as on the in vitro testing of the antioxidant activity. Study results showed that neochlorogenic and chlorogenic acids (5.2 and 4.85 mg/g dry weight, respectively) were the most abundant phenolic compounds in the flowers. (-)-Epicatechin and procyanidin B2 were the most abundant phenolic compounds in leaves and fruits. The highest content of phenolic compounds was found in leaves harvested in the early maturity stages, with a tendency of declining in the later stages of maturity. On the other hand, in the case of fruits, the most noticeable changes were found in the content of flavan-3-ols. The highest content of (-)-epicatechin (21.1 mg/g fresh weight) in fruit was in August, and those of procyanidins B2 and B5 in September (10.6 and 3.74 mg/g fresh weight, respectively). Statistical analysis showed a strong correlation between the total phenolics content and the antioxidant activity. This study has demonstrated that there is seasonal variation in both the quantity and the type of phenolic compound as well as the antioxidant activity of different parts of hawthorn.
FUNDING
The research was financed by the Ministry of Education and Science, the Republic of Serbia, projects No. TR 31060 and 45022 internal macro project of Faculty of Sciences and Mathematics, Kosovska Mitrovica.
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