TEMPO®SYSTEM IN MICROBIOLOGICAL ANALYSIS OF SPICES AND FOOD ADDITIVES

Thanks to the research it was possible to evaluate how useful is the automated TEMPO^® system (bioMerieux) for determining the total number of mesophilic microorganisms in spices and food additives, characterised by a high concentration of dyes. Statistical analysis of the results by linear regression proves the equivalence of the TEMPO^®method and the standard count plate method, at a correlation coefficient of 0.99. According to the findings, the dye of the examined matrices does not influence the result of the fluorescent signal at sample dilution of 1/4 000, which results in microbiological matrices contamination, not lower than 1.0 x 10³ CFU/g. In the case of colourful food matrices demonstrating a high level of contamination with mesophilic microflora, such as whole black peppercorns (the level of microflora above 4.9 x 10⁶ CFU/g), sample dilutions of 1/40 000 should be applied.

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eISSN:	2083-6007
ISSN:	1230-0322

W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.