ELECTROPHORETIC ISOFOCUSING IN STUDIES ON MEAT PROTEIN FROM VARIOUS ANIMAL SPECIES AND PORK STORED UNDER DIFFERENT CONDITIONS
 
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Pol. J. Food Nutr. Sci. 2005;55(3):299–302
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ABSTRACT:
The aim of investigations was to evaluate the IEF separation patterns of proteins from meat of various animal species using direct contact electrophoresis (DCE) on agarose gel. These separation patterns were compared with the results of electrophoresis of meat centrifugal drip after deionization. All separations were accompanied by immunoblotting against titin. The comparison of IEF separations of proteins from meat of various animal species by DCE revealed that their patterns were similar to those of proteins from the centrifugal drip, although they were slightly more corrugated and of a more varied width. Changes in proteins from various fractions of the centrifugal drip of pork stored for 168 h were also analysed. The comparison of samples from measurements performed 48 and 168 h after slaughter revealed that, during meat storage, not only did the number of proteins in the drip increase but their IP also changed. As a rule, titin degradation products showed higher IP values.