PCR–BASED DNA TESTS FOR DETECTION OF EMETIC BACILLUS CEREUS STRAINS PRODUCING CEREULIDE
 
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Pol. J. Food Nutr. Sci. 2007;57(Special issue 3A):101–105
 
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ABSTRACT
Two PCR-based methods for identification of emetic toxin producing Bacillus cereus strains were developed. The first set of primers cesBF and cesBR allowed for the amplification of cesBI 838 bp long fragment of cereulide biosynthesis operon for cereulide producing strains, or 421 bp long fragment of nonribosomal peptide synthetase (nrps) for emetic toxin non producing strains. Detection of both genes cesBI and nrps was possible in one PCR reaction. Among 24 strains of Cereus group tested, only one named 19W-cesB contained cesBI gene fragment. Bacillus cereus isolate 19W-cesB did not contain of any other genes of nonribosomal peptide synthetases responsible for the synthesis of other low molecular weight peptide toxins. The shdR and shdF second primer set allowed for specific amplification of the other 690 bp long fragment of cesBII gene. Only strain 19W-cesB allowed for the PCR synthesis of appropriate amplicon from all tested strains. Proposed methods may be fast and reliable techniques for detection of Bacillus cereus strains producing cereulide.
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