Search for Author, Title, Keyword
Role of Monophenols in the Recovery Process of Wild-Type Yeast Cells Subjected to Severe Environmental Stress
More details
Hide details
Publication date: 2013-09-30
Pol. J. Food Nutr. Sci. 2013;63(3):187–191
Phenolic compounds are important plant metabolites associated with the plant's defence reactions in conditions of biotic and abiotic stress. Heterotrophic organisms are not capable of synthesizing phenolic compounds, but take them up with foods of plant origin. Many of them exhibit antioxidant activity in chemical and biological assays. This study tested whether these compounds are able to rescue cells of a heterotrophic organism from the effects of severe environmental stress. A wild-type strain of Saccharomyces cerevisiae yeast was used for the experiments. Yeast cells that were pre-incubated in conditions of severe thermal, osmotic or oxidative stress and then plated on a medium enriched with benzoic acid and its derivatives had similar survival to cells growing on a medium with no supplements, while in the presence of cinnamic acid derivatives and coumarin a reduction in the survival of these cells was observed. In the presence of the synthetic monophenol BHT, two different reactions were noted – a decease in survival after 10 minutes of severe heat shock, and similar survival to that of the cells growing on an unsupplemented medium after 5 minutes of heat shock and the other types of stress. The results obtained suggest that monophenols used after severe environmental stress are not capable of eliminating the detrimental effects of these factors.
Application of growth tests employing a Δ sod1 mutant of Saccharomyces cerevisiae to study the antioxidant activity of berry fruit extracts
Agata Święciło, Kamila Rybczyńska-Tkaczyk, Agnieszka Najda, Anna Krzepiłko, Roman Prażak, Grażyna Zawiślak