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ORIGINAL ARTICLE
Tumor Anti-Initiation and Anti-Progression Properties of Sulphated-Extract of Colocasia esculenta
| 1 | Clinical Laboratory Sciences Department, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia |
| 2 | Department of Natural and Microbial Products Chemistry, National Research Centre, Dokki, Cairo 12622, Egypt |
| 3 | Cancer Biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, 33 El Buhouth St. Dokki, Cairo, 12622, Egypt |
| 4 | Biochemistry Department, National Research Centre, 33 El Buhouth St. Dokki, Cairo, 12622, Egypt |
| 5 | Radiological Sciences Department, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia |
CORRESPONDING AUTHOR
Amira M. Gamal-Eldeen
Clinical Laboratory Sciences Department, College of Applied Medical Sciences, Taif University, Saudi Arabia
Clinical Laboratory Sciences Department, College of Applied Medical Sciences, Taif University, Saudi Arabia
Submission date: 2021-05-25
Final revision date: 2021-10-01
Acceptance date: 2021-10-04
Online publication date: 2021-10-26
Publication date: 2021-10-26
Pol. J. Food Nutr. Sci. 2021;71(4):393–401
KEYWORDS
Colocasia esculentaCYP1Acancer chemoprevention & tumor anti-initiatingHDACmacrophage functionbreast MCF-7 carcinoma
TOPICS
ABSTRACT
Colocasia esculenta (Taro) is an edible tuberous plant; however, corms are its most worldwide consumed part while the corm powder is widely used in food industries. In this work, a sulphated polysaccharide extract of C. esculenta corm (SCE) was prepared and its cancer chemopreventive properties were explored. The amending of carcinogen metabolism and radical scavenging affinity revealed that SCE is a strong tumor anti-initiation agent via suppressing cytochrome P450-1A and enhancing glutathione and the carcinogen detoxification enzyme; glutathione S-transferase. SCE exhibited a strong scavenging affinity towards critical radicals (hydroxyl and peroxyl). It induced lymphocyte growth and modulated the macrophage functions into an anti-inflammatory profile, via elevating macrophage proliferation and its binding affinity of fluorescein isothiocyanate-lipopolysaccharide (FITC-LPS) and inhibiting nitric oxide and tumor necrosis factor-α generation. Furthermore, SCE showed a potent cytotoxicity against human breast MCF-7 carcinoma cells (IC50 27.73 µg/mL), whereas SCE treatment inhibited the activity of histone deacetylase (HDAC IC50 37.70 µg/mL) and disturbed the pattern of cell cycle phases. An arrest in both S- and G2/M-phases was linked with shifted cell populations towards late apoptosis and necrosis, as detected by flow cytometry. SCE is a promising cancer chemopreventive agent to be used in healthy food industries and for the high breast cancer-risk population.
ACKNOWLEDGEMENTS
The authors gratefully acknowledge the support of the Deanship of Scientific Research, Taif University.
FUNDING
This work was supported by Taif University Researchers Supporting Project Number (TURSP-2020/103).
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CITATIONS (1):
1.
Preparation, structural characteristics, and application of taro polysaccharides in food
Wenting Zhang, Gangliang Huang
Journal of the Science of Food and Agriculture
Wenting Zhang, Gangliang Huang
Journal of the Science of Food and Agriculture
Digitalization of scientific manuscripts published in PJFNS quarterly to enable open access to them in the Internet – task financed under the agreement no. 607/P-DUN/2019 from funds of the Minister of Science and Higher Education intended for science dissemination activities.
Utrzymanie procedur zabezpieczających oryginalność artykułów naukowych publikowanych w kwartalniku PJFNS - zadanie finansowane w ramach umowy 607/P-DUN/2019 ze środków Ministra Nauki i Szkolnictwa Wyższego przeznaczonych na działalność upowszechniającą naukę.
Maintenance of procedures assuring originality of scientific manuscripts published in PJFNS quarterly – task financed under the agreement no. 607/P-DUN/2019 from funds of the Minister of Science and Higher Education intended for science dissemination activities.
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