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ORIGINAL ARTICLE
Tumor Anti-Initiation and Anti-Progression Properties of Sulphated-Extract of Colocasia esculenta
| 1 | Clinical Laboratory Sciences Department, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia |
| 2 | Department of Natural and Microbial Products Chemistry, National Research Centre, Dokki, Cairo 12622, Egypt |
| 3 | Cancer Biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, 33 El Buhouth St. Dokki, Cairo, 12622, Egypt |
| 4 | Biochemistry Department, National Research Centre, 33 El Buhouth St. Dokki, Cairo, 12622, Egypt |
| 5 | Radiological Sciences Department, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia |
CORRESPONDING AUTHOR
Amira M. Gamal-Eldeen
Clinical Laboratory Sciences Department, College of Applied Medical Sciences, Taif University, Saudi Arabia
Clinical Laboratory Sciences Department, College of Applied Medical Sciences, Taif University, Saudi Arabia
Submission date: 2021-05-25
Final revision date: 2021-10-01
Acceptance date: 2021-10-04
Online publication date: 2021-10-26
Publication date: 2021-10-26
KEYWORDS
Colocasia esculentaCYP1Acancer chemoprevention & tumor anti-initiatingHDACmacrophage functionbreast MCF-7 carcinoma
TOPICS
ABSTRACT
Colocasia esculenta (Taro) is an edible tuberous plant; however, corms are its most worldwide consumed part while the corm powder is widely used in food industries. In this work, a sulphated polysaccharide extract of C. esculenta corm (SCE) was prepared and its cancer chemopreventive properties were explored. The amending of carcinogen metabolism and radical scavenging affinity revealed that SCE is a strong tumor anti-initiation agent via suppressing cytochrome P450-1A and enhancing glutathione and the carcinogen detoxification enzyme; glutathione S-transferase. SCE exhibited a strong scavenging affinity towards critical radicals (hydroxyl and peroxyl). It induced lymphocyte growth and modulated the macrophage functions into an anti-inflammatory profile, via elevating macrophage proliferation and its binding affinity of fluorescein isothiocyanate-lipopolysaccharide (FITC-LPS) and inhibiting nitric oxide and tumor necrosis factor-α generation. Furthermore, SCE showed a potent cytotoxicity against human breast MCF-7 carcinoma cells (IC50 27.73 µg/mL), whereas SCE treatment inhibited the activity of histone deacetylase (HDAC IC50 37.70 µg/mL) and disturbed the pattern of cell cycle phases. An arrest in both S- and G2/M-phases was linked with shifted cell populations towards late apoptosis and necrosis, as detected by flow cytometry. SCE is a promising cancer chemopreventive agent to be used in healthy food industries and for the high breast cancer-risk population.
ACKNOWLEDGEMENTS
The authors gratefully acknowledge the support of the Deanship of Scientific Research, Taif University.
FUNDING
This work was supported by Taif University Researchers Supporting Project Number (TURSP-2020/103).
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