Effect of Dietary Fibre Fractions on In Vitro Digestibility of Rapeseed Napin Proteins

Protein digestibility may be influenced by the presence of dietary fibre affecting the nutritional quality of a feed or food product. This study investigated the interplay between rapeseed (Brassica napus L.) protein and fibre constituents separated by industrially scalable pilot plant processing and recombined in mixed samples. Total dietary fibre (TDF) fractions were isolated from rapeseed hulls (TDF-RH) and purified rapeseed embryo fibres (TDF-RE). The effect of TDF sources on in vitro protein digestibility (IVPD) of a rapeseed protein concentrate rich in napin proteins (RP2) was assessed at three inclusion levels (200, 333, and 500 mg/g DM) using a sequential transient proteolysis by pepsin (1 h) and pancreatin (1 h). The IVPD of RP2 was dose dependently decreased upon addition of hull fibres at all inclusion levels (8.9 26.6%; P<0.05), whereas the effect of embryo fibres was of a markedly lower magnitude and only significant at the medium to high levels (7.3 8.9%; P<0.05). These results demonstrated that TDF fractions obtained from rapeseed differentially affect the protein digestibility of rapeseed napin proteins depending on the fibre source and inclusion level.

REFERENCES (39)

Acton J.C., Breyer L., Satterlee L.D., Effect of dietary fiber constituents on the in vitro digestibility of casein. J. Food Sci., 1982, 47, 556–560.

Google Scholar

Andersen K.E., Bagger C., Sørensen H., Sørensen J.C., A process for the manufacture of products from cruciferous crops. Denmark, Patent no. WO/2012/149941, 2012, Available from: [https://patents.google.com/pat...].

Google Scholar

AOAC International, Official Method 985.29. Total dietary fiber in foods. 1985, in: Official Methods of Analysis of AOAC International, Gaithersburg, MD, USA.

Google Scholar

AOAC International, Official Method 950.46 (Modified). Moisture in meat. 2000, in: Official Methods of Analysis of AOAC International, Gaithersburg, MD, USA.

Google Scholar

Asp N.G., Johansson C.G., Hallmer H., Siljeström M., Rapid enzymatic assay of insoluble and soluble dietary fiber. J. Agric. Food Chem., 1983, 31, 476–482.

Google Scholar

Bjergegaard C., Eggum B.O., Jensen S.K., Sørensen H., Dietary fibres in oilseed rape: Physiological and antinutritional effects in rats of isolated IDF and SDF added to a standard diet. J. Anim. Physiol. Anim. Nutr., 1991a, 66, 69–79.

Google Scholar

Bjergegaard C., Jensen S.K., Sørensen H., Dietary fibres in oilseed rape: Properties and effects on the digestibility of rapeseed meal. GCIRC Congr. Saskat. Can., 1991b, 2, 448–453.

Google Scholar

Bjergegaard C., Sørensen H., Sørensen S., Dietary fibres and associated compounds in rape seed and biorefined rape seed products compared to DF in pea. J. Anim. Feed Sci., 1997, 6, 163–184.

Google Scholar

Bos C., Airinei G., Mariotti F., Benamouzig R., Bérot S., Evrard J., Fénart E., Tomé D., Gaudichon C., The poor digestibility of rapeseed protein is balanced by its very high metabolic utilization in humans. J. Nutr., 2007, 137, 594–600.

Google Scholar

10.

Brulé D., Savoie L., In vitro digestibility of protein and amino acids in protein mixtures. J. Sci. Food Agric., 1988, 43, 361–372.

Google Scholar

11.

Campbell L., Rempel C.B., Wanasundara J.P.D., Canola/rapeseed protein: Future opportunities and directions — Workshop proceedings of IRC 2015. Plants, 2016, 5, art. no. 17.

Google Scholar

12.

Danielsen V., Eggum B.O., Jensen S.K., Sørensen H., Dehulled protein-rich rapeseed meal as a protein source for early weaned piglets. Anim. Feed Sci. Technol., 1994, 46, 239–250.

Google Scholar

13.

Dutta S.K., Hlasko J., Dietary fiber in pancreatic disease: effect of high-fiber diet on fat malabsorption in pancreatic insufficiency and in vitro study of the interaction of dietary fiber with pancreatic enzymes. Am. J. Clin. Nutr., 1985, 41, 517–525.

Google Scholar

14.

Fleddermann M., Fechner A., Rößler A., Bähr M., Pastor A., Liebert F., Jahreis G., Nutritional evaluation of rapeseed protein compared to soy protein for quality, plasma amino acids, and nitrogen balance – A randomized cross-over intervention study in humans. Clin. Nutr., 2013, 32, 519–526.

Google Scholar

15.

Gagne C.M., Acton J.C., Fiber constituents and fibrous food residue effects on the in vitro enzymatic digestion of protein. J. Food Sci., 1983, 48, 734–738.

Google Scholar

16.

Goodman B.E., Insights into digestion and absorption of major nutrients in humans. Adv. Physiol. Educ., 2010, 34, 44–53.

Google Scholar

17.

Grala W., Verstegen M.W., Jansman A.J., Huisman J., van Leeusen P., Ileal apparent protein and amino acid digestibilities and endogenous nitrogen losses in pigs fed soybean and rapeseed products. J. Anim. Sci., 1998, 76, 557–568.

Google Scholar

18.

Grundy M.M.-L., Edwards C.H., Mackie A.R., Gidley M.J., Butterworth P.J., Ellis P.R., Re-evaluation of the mechanisms of dietary fibre and implications for macronutrient bioaccessibility, digestion and postprandial metabolism. Br. J. Nutr., 2016, 116, 816–833.

Google Scholar

19.

Hansen W.E., Effect of dietary fiber on proteolytic pancreatic enzymes in vitro. Int. J. Pancreatol., 1986, 1, 341–351.

Google Scholar

20.

Ivanova P., Chalova V., Uzunova G., Koleva L., Manolov I., Biochemical characterization of industrially produced rapeseed meal as a protein source in food industry. Agric. Agric. Sci. Procedia, 2016, 10, 55–62.

Google Scholar

21.

Joehnke M.S., Rehder A., Sørensen S., Bjergegaard C., Sørensen J.C., Markedal K.E., In vitro digestibility of rapeseed and bovine whey protein mixtures. J. Agric. Food Chem., 2018, 66, 711–719.

Google Scholar

22.

Khajali F., Slominski B.A., Factors that affect the nutritive value of canola meal for poultry. Poult. Sci., 2012, 91, 2564–2575.

Google Scholar

23.

Kracht W., Dänicke S., Kluge H., Keller K., Matzke W., Hennig U., Schumann W., Effect of dehulling of rapeseed on feed value and nutrient digestibility of rape products in pigs. Arch. Anim. Nutr., 2004, 58, 389–404.

Google Scholar

24.

Mansour E.H., Dworschák E., Lugasi A., Gaál Ö., Barna É., Gergely A., Effect of processing on the antinutritive factors and nutritive value of rapeseed products. Food Chem., 1993, 47, 247–252.

Google Scholar

25.

Mosenthin R., Sauer W.C., Ahrens F., Dietary pectin’s effect on ileal and fecal amino acid digestibility and exocrine pancreatic secretions in growing pigs. J. Nutr., 1994, 124, 1222–1229.

Google Scholar

26.

Ochodzki P., Rakowska M., Rek-Cieply B., Bjergegaard C., Sørensen H., Studies on enzymatic fractionation, chemical composition and biological effects of dietary fibre in rape seed (Brassica napus L. ). 2. Influence of rape seed dietary fibre on digestibility of protein and organic matter using unprocessed and heated full fat rape seed and isolated dietary fibre fractions added to rat diets. J. Anim. Feed Sci., 1995, 4, 139–151.

Google Scholar

27.

Pasha I., Saeed F., Sultan M.T., Khan M.R., Rohi M., Recent developments in minimal processing: A tool to retain nutritional quality of food. Crit. Rev. Food Sci. Nutr., 2014, 54, 340–351.

Google Scholar

28.

Ren L.Q., Zhao F., Tan H.Z., Zhao J.T., Zhang J.Z., Zhang H.F., Effects of dietary protein source on the digestive enzyme activities and electrolyte composition in the small intestinal fluid of chickens. Poult. Sci., 2012, 91, 1641–1646.

Google Scholar

29.

Richter C.K., Skulas-Ray A.C., Champagne C.M., Kris-Etherton P.M., Plant protein and animal proteins: Do they differentially affect cardiovascular disease risk?. Adv. Nutr., 2015, 6, 712–728.

Google Scholar

30.

Saunders R.M., Betschart A.A., The significance of protein as a component of dietary fiber. Am. J. Clin. Nutr., 1980, 33, 960–961.

Google Scholar

31.

Savoie L., Galibois I., Parent G., Charbonneau R., Sequential release of amino acids and peptides during in vitro digestion of casein and rapeseed proteins. Nutr. Res., 1988, 8, 1319–1326.

Google Scholar

32.

Schulze H., van Leeuwen P., Verstegen M.W., Huisman J., Souffrant W.B., Ahrens F., Effect of level of dietary neutral detergent fiber on ileal apparent digestibility and ileal nitrogen losses in pigs. J. Anim. Sci., 1994, 72, 2362–2368.

Google Scholar

33.

Shah N., Atallah M.T., Mahoney R.R., Pellett P.L., Effect of dietary fiber components on fecal nitrogen excretion and protein utilization in growing rats. J. Nutr., 1982, 112, 658–666.

Google Scholar

34.

Slominski B.A., Jia W., Rogiewicz A., Nyachoti C.M., Hickling D., Low-fiber canola. Part 1. Chemical and nutritive composition of the meal. J. Agric. Food Chem., 2012, 60, 12225–12230.

Google Scholar

35.

Stone A.K., Teymurova A., Dang Q., Abeysekara S., Karalash A., Nickerson M.T., Formation and functional attributes of electrostatic complexes involving napin protein isolate and anionic polysaccharides. Eur. Food Res. Technol., 2014, 238, 773–780.

Google Scholar

36.

Tan S.H., Mailer R.J., Blanchard C.L., Agboola S.O., Canola proteins for human consumption: Extraction, profile, and functional properties. J. Food Sci., 2011, 76, R16–R28.

Google Scholar

37.

USDA FAS, Oilseeds: World Markets and Trade, 2018, Available from: [https://apps.fas.usda.gov/psdo.... Last accessed 04/06/2018].

Google Scholar

38.

Wanasundara J.P.D., Proteins of Brassicaceae oilseeds and their potential as a plant protein source. Crit. Rev. Food Sci. Nutr., 2011, 51, 635–677.

Google Scholar

39.

Zhang B., Liu G., Ying D., Sanguansri L., Augustin M.A., Effect of extrusion conditions on the physico-chemical properties and in vitro protein digestibility of canola meal. Food Res. Int., 2017, 100, 658–664.

Google Scholar

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