Sulphoraphane (SF) is isothiocyanate that is present naturally in widely consumed vegetables and has been shown to block the formation of many kinds of tumors. The effect of sulphoraphane, administered intragastrically (10 mg/kg) once a day for 2 weeks, on some metabolic and antioxidative parameters in water- and ethanol-drinking (10% ethanol solution) rats was investigated. Its protective effect was previously observed, but its influence on metabolic changes has not been yet characterised. Our trial confirmed antioxidative properties of sulphoraphane by limited generation of lipid peroxidation and enhanced functional capacity of detoxifying enzyme glutathione S-transferase (GST). SF in water-drinking rats caused a decrease in malondialdehyde (TBARS) and glutathione peroxidase (GPx) activity, whereas glutathione S-transferase (GST) activity was augmented significantly. However, we observed unfavourable interaction between sulphoraphane and ethanol, where antioxidative properties of SF were abolished in ethanol-drinking rats. The present trial was the first attempt to determinate the carbohydrate and lipid metabolism changes after SF administration in both afore-mentioned conditions. We found that SF per se caused insulinaemia, steatosis as well as beneficial HDL-cholesterolaemia and serum triacylglycerols drop. Under ethanol treatment we noted the following disturbances: increased triacylglycerols and drop of glycogen and TBARS content in the liver. Such characteristics unfavourably elevated liver level of triacylglycerols was not mitigated by sulphoraphane. The other ethanol disturbances were normalized by SF. In our trial we noted clear interaction between sulphoraphane and ethanol. Liver glycogen decrease caused by ethanol and serum insulin increment due to sulphoraphane ingestion was normalized by their interaction. Serum triacylglycerols and HDL-cholesterol changes caused by SF per se were maintained also after sulphoraphane treatment in the ethanol-receiving rats. However, after their interaction, the content of triacylglycerols in the liver did not increase strongly in spite of significant increment during their individual action. The results obtained demonstrate that SF exerts an influence on insulin level as well as lipid metabolism. Only insulin or glycogen disturbance caused by sole SF or ethanol was normalized during their interaction. SF does not attenuate ethanol steatosis. Additionally, our trial showed that the confirmed antioxidative properties of SF were abolished in the presence of ethanol.