A Spectrophotometric Assay for Total Garlic Thiosulfinates Content. Kinetic Aspects of Reaction with Chromogenic Thiols.
 
More details
Hide details
Publication date: 2012-03-31
 
Pol. J. Food Nutr. Sci. 2012;62(1):23–29
 
KEYWORDS
ABSTRACT
Garlic (Allium sativum) is a plant known for its antimicrobial, antithrombotic and antiatherosclerotic properties. Those features are attributed to thiosulfinates, compounds formed in an enzymatic reaction occurring during garlic cloves crashing. A spectrophotometric method for the determination of thiosulfinate concentration in garlic extracts and supplements was proposed. It is based on reactions between thiosulfinates and selected chromogenic thiol compounds: 2 mercaptopyridine (2 MP), 4 mercaptopyridine (4 MP), 1 oxide 2 mercaptopyridine (MPO), 2 mercaptopyrimidine (MPM) and 2 nitro 5 thiobenzoic acid (NTB). Then kinetics of the reactions was studied. Rate constants and orders were determined. All reactions are first order with respect to the thiol and first order with respect to TS so the reactions of all tested thiols with garlic TS run in accordance with second order kinetics. Reaction rates vary among the thiol compounds: the constant rates differ from 6.7 (mol/L)-1.min-1 for 2 MP to 1129 (mol/L)-1.min-1 for NTB. Knowledge of the kinetic equation for the reactions and measurements of the initial rates allowed determining thiosulfinate contents in garlic extracts and supplements.
 
CITATIONS (8):
1.
Identifying Thermal and pH Stability of Thiols Compound in Garlic Extract
Sity Samsudin, Nurlidia Mansor, Suriati Sufian, Zakaria Man
Applied Mechanics and Materials
 
2.
Synthesis and Characterization of Rotary Forcespun Polyvinylpyrrolidone Fibers Loaded by Garlic (Allium sativum) Extract
Fathiyah Zahra, Ahmad Fauzi, Munir Miftahul, Khairurrijal Khairurrijal
IOP Conference Series: Materials Science and Engineering
 
3.
Analytical methods for bioactive sulfur compounds in Allium: An integrated review and future directions
Daniela Ramirez, Daniela Locatelli, Roxana González, Pablo Cavagnaro, Alejandra Camargo
Journal of Food Composition and Analysis
 
4.
Synthesis of HA-SS-MP: A Prodrug With High Specificity for Cancer Cells
Bo Tao, Zong-ning Yin
Natural Product Communications
 
5.
Recent Applications and Potential of Infrared Dryer Systems for Drying Various Agricultural Products: A Review
Fakhreddin Salehi
International Journal of Fruit Science
 
6.
Thiosulfinates Concentration in Garlic Extract at Different Storage Temperatures
Sity Samsudin, Nurlidia Mansor, Suriati Sufian, Zakaria Man
Applied Mechanics and Materials
 
7.
The Potential of Garlic Extract as Bio-Inhibitor in Urea Fertilizer
Sity Samsudin, Mansor Binti, Suriati Sufian, Zakaria Man
Key Engineering Materials
 
8.
Quantification and Characterization of Allicin in Garlic Extract
Nurlidia Mansor, Ho Herng, Sity Samsudin, Suriati Sufian, Yoshimitsu Uemura
Journal of Medical and Bioengineering
 
eISSN:2083-6007
ISSN:1230-0322