CHROMATOGRAPHIC SEPARATION OF PHENOLIC COMPOUNDS FROM RAPESEED BY SE-HPLC – A SHORT REPORT
 
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Pol. J. Food Nutr. Sci. 2007;57(1):87–90
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ABSTRACT:
Phenolic compounds from rapeseed were extracted into 80% (v/v) methanol. The crude extract was then separated using size exclusion high-performance liquid chromatography (SE-HPLC) on a TSK G2000SWXL column with a mobile phase of 45% acetonitrile and 0.1% TFA (v/v). Six peaks were identified on the chromatogram: the main peak originated from sinapin. Calibration of the assay was characterised by an excellent linearity of the sinapin standard (r2 = 1.0). The method so described can be used for screening phenolic constituents in rapeseed or canola. It is also useful for the purification of the sinapin as a standard from the crude extract.