COMPARISON OF PHYSIOLOGICAL AND PCR-RFLP RDNA IDENTIFICATION OF YEAST SPECIES COMMONLY FOUND IN CHEESE
 
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Pol. J. Food Nutr. Sci. 2007;57(2):221–226
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ABSTRACT
The identification of twenty four yeast isolates from cheese performed with API ID 32C simplified assimilation test and restriction fragments length polymorphism (PCR-RFLP) of ITS1-5.8S rDNA-ITS2 region was compared. The accordance of the results of both methods for eleven strains identified as Candida famata, five strains as C. lipolytica and four strains as C. sphaerica was observed. However, the other four yeast isolates classified with API as C. sphaerica revealed amplification products as well as restriction patterns characteristic for C. famata species.
ISSN:1230-0322