ORIGINAL ARTICLE
Cytoprotective Effect of Morchella esculenta Protein Hydrolysate and Its Derivative Against H2O2-Induced Oxidative Stress
Qiang Zhang 1, 2, 3  
,  
Caie Wu 1, 3  
,  
Yujun Sun 2  
,  
Tingting Li 1, 3  
,  
Gongjian Fan 1, 3  
 
 
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1
College of Light Industry and Food Engineering, Nanjing Forestry University, Nanjing 210037, China
2
College of Life and Health Sciences, Anhui Science and Technology University, Fengyang 233100, China
3
Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, Nanjing 210037, China
CORRESPONDING AUTHOR
Caie Wu   

Co-Innovation Center for Sustainable Forestry in Southern China, Nanjing Forestry University, China
Online publish date: 2019-06-28
Submission date: 2019-04-16
Final revision date: 2019-06-12
Acceptance date: 2019-06-19
 
KEYWORDS
TOPICS
ABSTRACT
Morchella protein hydrolysate (MPH) and its glycosylated derivative (G-MPH) may possess the potential as natural antioxidants. However, knowledge about the protective effects of MPH and G-MPH on cellular oxidative damage is limited. This study evaluated whether MPH and G-MPH protected Caco-2 cells from H2O2-induced oxidative injury and explored the potential mechanisms of protection. The results showed that, under H2O2 stress, both MPH and G-MPH significantly increased cell viability, suppressed intracellular ROS and MDA production, increased cellular antioxidant capacity, and activated Nrf2 signaling pathway. More importantly, MPH and G-MPH significantly inhibited the H2O2-induced apoptosis via restoring the loss of mitochondrial membrane potential and regulating the protein expressions of Bax, Bcl-2, and caspase-3. These data indicate that MPH and G-MPH can protect Caco-2 cells against oxidative injury by improving cellular antioxidant responses and inhibiting apoptosis. Therefore, MPH and G-MPH can have a broad application potential as promising ingredients of nutraceutical products or functional foods.
FUNDING
This work was supported by the grant from the Doctorate Fellowship Foundation of Nanjing Forestry University (2014), the Natural Science Foundation of Anhui Provincial Department of Education (Project No. KJ2017A515), the Postgraduate Research & Practice Innovation Program of Jiangsu Province (Project No. KYLX15_0916), the Natural Science Foundation of Jiangsu province (Project No. BK20150883), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).
 
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