HIGH PERFORMANCE LIQUID CHROMATOGRAPHY ANALYSIS OF REDUCED GLUTATHIONE IN BIOLOGICAL MATERIALS.

A reverse-phase HPLC spectrofluorometric method was developed for routine, specific and fast analysis of glutathione (GSH) in biological materials based on modified O-opthaldialdehyde derivatization procedures. The developed method allowed the determination of glutathione in yeast biomass, bile and plasma samples from goats. The limit of detection was estimated to be 8 µM for yeast biomass at a signal to noise ratio of 1 and was further extended to signal ratio of 2, which allowed the detection of lower amounts of GSH in the bile and plasma up to 10 ng/cm3. The methods of GSH extraction from biological materials, derivatization procedures and separation by HPLC method were reported.

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eISSN:	2083-6007
ISSN:	1230-0322

W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.