HIGH PERFORMANCE LIQUID CHROMATOGRAPHY ANALYSIS OF REDUCED GLUTATHIONE IN BIOLOGICAL MATERIALS.
 
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Pol. J. Food Nutr. Sci. 2001;51(1):31–35
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ABSTRACT
A reverse-phase HPLC spectrofluorometric method was developed for routine, specific and fast analysis of glutathione (GSH) in biological materials based on modified O-opthaldialdehyde derivatization procedures. The developed method allowed the determination of glutathione in yeast biomass, bile and plasma samples from goats. The limit of detection was estimated to be 8 µM for yeast biomass at a signal to noise ratio of 1 and was further extended to signal ratio of 2, which allowed the detection of lower amounts of GSH in the bile and plasma up to 10 ng/cm3. The methods of GSH extraction from biological materials, derivatization procedures and separation by HPLC method were reported.
ISSN:1230-0322