IMMOBILISATION OF THE PROTEOLYTIC ENZYME SYSTEM (CHYMOTRYPSIN-EXOPEPTIDASE) IN HIGH-MOLECULAR POLYSACCHARIDES.
 
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Pol. J. Food Nutr. Sci. 2000;50(1):43–46
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ABSTRACT
Three kinds of high-molecular polysaccharides alginate, chitosan and carrageenan were tested as carriers for the immobilisation of proteolytic enzymes. The catalytic system consisted of the endopeptidase - chymotrypsin and exopeptidases: carboxypeptidase A and leucine aminopeptidase. Subsequently, this immobilised system was used for making of enzymatic hydrolysates of casein. In the case of periodic method of casein hydrolysis the best results (71.9% of tyrosine and tryptophan released) were achieved after 72 h, at substrate concentration 0.5% and with alginate as a carrier. In the semi-continuous method chitosan appeared to be the best carrier and after 72 h the degree of hydrolysis was 48.6% (52.7% of tyrosine and tryptophan released) at the concentration of casein 2%.
ISSN:1230-0322