Influence of Polyphenol Extract from Evening Primrose (Oenothera Paradoxa) Seeds on Proliferation of Caco-2 Cells and on Expression, Synthesis and Activity of Matrix Metalloproteinases and Their Inhibitors
More details
Hide details
Publication date: 2014-09-30
Pol. J. Food Nutr. Sci. 2014;64(3):181–191
Evening primrose (Oenothera paradoxa Hudziok) seeds are a rich source of not only a valuable oil containing an essential fatty acid - γ-linolenic acid (GLA) - but also polyphenols which can be obtained from the biomass remaining after oil pressing. The aim of our studies was to evaluate the influence of a polyphenol extract from defatted seeds of evening primrose on human colorectal adenocarcinoma Caco-2 cell proliferation and matrix metalloproteinases (MMPs) synthesis and activity. To assess the effect of evening primrose extract on the proliferation crystal violet staining and sulforhodamine B (SRB) assays were used whereas mRNA expression and activity of MMPs were evaluate by RT-PCR and gelatin zymography. The results revealed that the examined polyphenol extract has little influence on Caco-2 proliferation, but effectively in a time- and dose-dependent manner inhibits MMP-1, MMP-7, MMP-9 and MMP-14 mRNA synthesis induced by TNF-α and TPA. Additionally, zymographic analysis revealed that after 24 h, the polyphenol extract at a concentration of 50 μmol/L GAE caused a 10-fold reduction in MMP-9 synthesis. Moreover, this extract might be a potent inhibitor of MMP activity. The results showed that polyphenol extract from evening primrose inhibited PBMC-derived MMP-2 and MMP-9 enzymatic activity in dose-dependent manner. The obtained results indicate that the polyphenol extract from evening primrose seeds could be an inhibitor of proteases involved in tumor progression and metastasis.
Interactions between procyanidin oligomers and the active form of matrix metalloproteinase‐7: A theoretical insight
Ana Mendoza‐Wilson, René Balandrán‐Quintana
International Journal of Quantum Chemistry
Moringa oleifera—Storage Stability, In Vitro-Simulated Digestion and Cytotoxicity Assessment of Microencapsulated Extract
Cecilia Castro-López, Catarina Gonçalves, Janeth Ventura-Sobrevilla, Lorenzo Pastrana, Cristóbal Aguilar-González, Guillermo Martínez-Ávila
Computational and Experimental Progress on the Structure and Chemical Reactivity of Procyanidins: Their Potential as Metalloproteinases Inhibitors
Ana Mendoza-Wilson, René Balandrán-Quintana
Current Organic Chemistry
Polyphenol composition and in vitro antiproliferative effect of corm, tepal and leaf from Crocus sativus L. on human colon adenocarcinoma cells (Caco-2)
Raúl Sánchez-Vioque, Omar Santana-Méridas, Moschos Polissiou, Javier Vioque, Konstantina Astraka, Manuel Alaiz, David Herraiz-Peñalver, Petros Tarantilis, Julio Girón-Calle
Journal of Functional Foods
Polyphenols Content, Antioxidant Activity, and Cytotoxicity Assessment of Taraxacum officinale Extracts Prepared through the Micelle-Mediated Extraction Method
Michał Miłek, Dana Marcinčáková, Jaroslav Legáth
Inhibition of nuclear factor-kappaB, cyclooxygenase-2, and metalloproteinase-9 expression by flavanols from evening primrose (Oenothera paradoxa) in human colon cancer SW-480 cells
Katarzyna Owczarek, Elżbieta Hrabec, Jakub Fichna, Dorota Sosnowska, Maria Koziołkiewicz, Jacek Szymański, Urszula Lewandowska
Journal of Functional Foods
Analysis of Cytotoxicity of Selected Asteraceae Plant Extracts in Real Time, Their Antioxidant Properties and Polyphenolic Profile
Patrycja Sowa, Dana Marcinčáková, Michał Miłek, Ewelina Sidor, Jaroslav Legáth, Małgorzata Dżugan