PRODUCTION OF BACTERIOCIN BY  CARNOBACTERIUM DIV ERGENS IN BATCH AND CONTINUOUS CULTURE

Bacteriocin production by Carnobacterium divergens was investigated in batch and continuous fermentation. The best batch bacteriocin productivity, obtained at 25 to 30°C and 2 % inoculum, was 2,580 AU cm^-3 h^-1 with no pH regulation and 68,270 AU cm^-3 h^-1 with pH controlled at 6.5. The cell division occurred faster at 37°C. Heat sterilization of broth the impaired biomass production but favoured the bacteriocin production. A large part of the bacteriocin molecules adsorbed on the cells after centrifugation and could be released to the medium by an acidic shock. Filtration of bacteriocin solution caused severe losses of activity. Continuous fermentation allowed to obtain a high bacteriocin productivity, reached 819,200 AU cm^-3, at dilution rate 0.12 h^-1 and biomass concentration 3.0 g dm^-3.

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eISSN:	2083-6007
ISSN:	1230-0322

W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.