EFFECT OF DIFFERENT INORGANIC NITROGEN SOURCES ON THE PRODUCTION OF CELLULASE-FREE β-1,4-XYLANASE BY  AUREOBASIDIUM PULLULANS

The effect of different levels of inorganic nitrogen sources (0.15, 0.21 and 0.25% nitrogen content) on the synthesis of cellulase-free β -1,4-xylanase by Aureobasidium pullulans DSM-73015 was examined. Among inorganic nitrogen compounds tested, ammonium nitrate (NH₄NO₃) was the best inorganic nitrogen source at 0.21% nitrogen content that gave the highest volumetric and specific activities (8830.09 nkat mL^-1; 10767.15 nkat/mg protein) after 96 h of cultivation, while urea displayed the lowest effect. Partial characterization of this crude enzyme produced after optimization processes on two different buffer systems showed that its optimum pH activity was 4, while the thermoactivity had its maximum at 50⁰C in both buffers. The highest volumetric and specific activities obtained were 20102.35 nkat mL^-1; 23000.40 nkat/mg protein for sodium acetate buffer and 19852.30 nkat mL^-1; 22714.31 nkat/mg protein for sodium citrate buffer.

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AN ATTEMPT TO APPLY A PULLULAN COATING TO REDUCE OXIDATIVE CHANGES AND MASS LOSS IN NUTS DURING STORAGE

PRODUCTION OF β-1,4-XYLANASE BY AUREOBASIDIUM PULLULANS DSM-73015: STIMULATORY EFFECT OF (EDTA) ETHYLENE DIAMINE TETRAACETIC ACID

IMPROVEMENT OF AUREOBASIDIUM PULLULANS A.P.-3 FOR PULLULAN BIOSYNTHESIS BY ASSOCIATED MUTAGENESIS.

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eISSN:	2083-6007
ISSN:	1230-0322

W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.