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PRODUCTION OF BACTERIOCIN BY CARNOBACTERIUM DIVERGENS IN BATCH AND CONTINUOUS CULTURE
Publication date: 1999-09-30
Pol. J. Food Nutr. Sci. 1999;49(3):27-38
KEYWORDS
ABSTRACT
Bacteriocin production by Carnobacterium divergens was investigated in batch and continuous fermentation. The best batch bacteriocin productivity, obtained at 25 to 30°C and 2 % inoculum, was 2,580 AU cm-3 h-1 with no pH regulation and 68,270 AU cm-3 h-1 with pH controlled at 6.5. The cell division occurred faster at 37°C. Heat sterilization of broth the impaired biomass production but favoured the bacteriocin production. A large part of the bacteriocin molecules adsorbed on the cells after centrifugation and could be released to the medium by an acidic shock. Filtration of bacteriocin solution caused severe losses of activity. Continuous fermentation allowed to obtain a high bacteriocin productivity, reached 819,200 AU cm-3, at dilution rate 0.12 h-1 and biomass concentration 3.0 g dm-3.
| eISSN: | 2083-6007 |
| ISSN: | 1230-0322 |
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