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Profile of Phenolic Acids and Flavonoids of Red Beet and Its Fermentation Products. Does Long-Term Consumption of Fermented Beetroot Juice Affect Phenolics Profile in Human Blood Plasma and Urine?
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Department of Chemistry and Biodynamics of Food, Division of Food Science, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima 10, 10-748 Olsztyn, Poland
Department of Human Nutrition, Faculty of Food Science, University of Warmia and Mazury in Olsztyn, Sloneczna 45f, 10-718 Olsztyn, Poland
Submission date: 2019-10-03
Final revision date: 2020-01-14
Acceptance date: 2020-01-15
Online publication date: 2020-02-12
Publication date: 2020-02-12
Corresponding author
Natalia Płatosz   

Department of Chemistry and Biodynamics of Food, Division of Food Science, Institute of Animal Reproduction and Food Research of Polish Academy of Sciences, Tuwima, 10-478, Olsztyn, Poland
Pol. J. Food Nutr. Sci. 2020;70(1):55–65
The aim of this study was to determine the profile of phenolic acids and flavonoids in red beetroot products and these compounds fate in the human body after long-term exposure to fermented red beet juice. Phenolic acids and flavonoids were analyzed by HPLC-MS/MS. The performed analysis revealed that beetroot and its products were the notable sources of phenolic acids and flavonoids, with isoferulic acid, protocatechuic acid, epicatechin, and apigenin predominating among the twenty compounds detected. However, the compounds analyzed appeared mainly in the bound form in the fresh material. The results obtained indicate that the fermentation process caused an increase in the content of free phenolic acids and reduced the content of conjugated phenolic acids. Contrarily to phenolic acids, the same process caused a reduction in the content of free flavonoids and an increase in the content of conjugated flavonoids. Moreover, the 14-day spontaneous fermentation process resulted in a significant reduction (by 45.18%) in the total content of phenolics (phenolic acids and flavonoids). However, it was observed that forty-two days of the regular intake of the fermented beetroot juice (200 mL/60 kg body weight) generally did not affect concentrations of phenolic acids and flavonoids in the volunteers' body fluids compared to their concentrations in fluids before fermented beetroot juice consumption.
This research was supported by the statutory funds of the Department of Chemistry and Biodynamics of Food of the Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn, Poland.
Acosta-Estrada, B.A., Gutiérrez-Uribe, J.A., Serna-Saldívar, S.O. (2014). Bound phenolics in foods: A review. Food Chemistry, 152, 46–55.
Aura, A.M., O'Leary, K.A., Williamson, G., Ojala, M., Bailey, M., Puupponen-Pimiä, R., Nuutila, A.M., Oksman-Caldentey, K.M., Poutanen, K. (2002). Quercetin derivatives are deconjugated and converted to hydroxyphenylacetic acids but not methylated by human fecal flora in vitro. Journal of Agricultural and Food Chemistry, 50(6), 1725-1730.
Ben Haj Koubaier, H., Snoussi, A., Essaidi, I., Chaabouni, M.M., Thonart, P., Bouzouita, N. (2014). Betalain and phenolic compositions, antioxidant activity of Tunisian red beet (Beta vulgaris L. conditiva) roots and stems extracts. International Journal of Food Properties, 17, 1934-1945.
Čanadanović-Brunet, J.M., Savatović, S.S., Ćetković, G.S., Vulić, J., Djilas, S., Sinisa, M., Cvetković, D.D. (2011). Antioxidant and antimicrobial activities of beet root pomace extracts. Czech Journal of Food Sciences, 29(6), 575-585.
Carrillo, C., Rey, R., Hendrickx, M., Del Mar Cavia, M., Alonso-Torre, S. (2017). Antioxidant capacity of beetroot: traditional vs novel approaches. Plant Foods for Human Nutrition, 72(3), 266-273.
Chhikara, N., Kushwaha, K., Sharma, P., Gat, Y., Panghal, A. (2019). Bioactive compounds of beetroot and utilization in food processing industry: A critical review. Food Chemistry, 272, 192–200.
Clifford, T., Howatson, G., West, D.J., Stevenson, E.J. (2015). The potential benefits of red beetroot supplementation in health and disease. Nutrients, 7(4), 2801-2822.
Değirmencioğlu, N., Gurbuz, O., Şahan, Y. (2016). The monitoring, via an in vitro digestion system, of the bioactive content of vegetable juice fermented with Saccharomyces cerevisiae and Saccharomyces boulardii. Journal of Food Processing and Preservation, 40(4), 798-811.
Feliciano, R.P., Istas, G., Heiss, C., Rodriguez-Mateos, A. (2016). Plasma and urinary phenolic profiles after acute and repetitive intake of wild blueberry. Molecules, 21(9), art. no. 1120.
Guiné, R.P.F., Gonçalves, F., Lerat, C., Idrissi, T.E., Rodrigi, E., Correia, P.M.R., Gonçalves, J.C. (2018). Extraction of phenolic compounds with antioxidant activity from beetroot (Beta vulgaris L.). Current Nutrition & Food Science, 14(4), 350-357.
Georgiev, V.G., Weber, J., Kneschke, E.M., Denev, P.N., Bley, T., Pavlov, A.I. (2010). Antioxidant activity and phenolic content of betalain extracts from intact plants and hairy root cultures of the red beetroot Beta vulgaris cv. Detroit dark red. Plant Foods for Human Nutrition, 65(2), 105-111.
Gonthier, M.P., Donovan, J.L., Texier, O., Felgines, C., Remesy, C., Scalbert, A. (2003). Metabolism of dietary procyanidins in rats. Free Radical Biology & Medicine, 35(8), 837-844.
Grosso, G., Stepaniak, U., Topor-Mądry, R., Szafraniec, K., Pająk, A. (2014). Estimated dietary intake and major food sources of polyphenols in the Polish arm of the HAPIEE study. Nutrition, 30(11-12), 1398-1403.
Guldiken, B., Toydemir, G., Nur Memis, K., Okur, S., Boyacioglu, D., Capanoglu, E. (2016). Home-processed red beetroot (Beta vulgaris L.) products: changes in antioxidant properties and bioaccessibility. International Journal of Molecular Sciences, 17(6), art. no. 858.
Hasan, M.N., Sultan, M.Z., Mar-E-Um, M. (2014). Significance of fermented food in nutrition and food science. Journal of Scientific Research, 6(2), 373-386.
Hunaefi, D., Akumo, D.N., Smetanska, I. (2013). Effect of fermentation on antioxidant properties of red cabbages. Food Biotechnology, 27(1), 66-85.
Hur, S.J., Lee, S.Y., Kim, Y.C., Choi, I., Kim, G.B. (2014). Effect of fermentation on the antioxidant activity in plant-based foods. Food Chemistry, 1(160), 346-356.
Huynh, N.T., Van Camp, J., Smagghe, G., Raes, K. (2014). Improved release and metabolism of flavonoids by steered fermentation processes: a review. International Journal of Molecular Sciences, 15(11), 19369-19388.
Kazimierczak, R., Hallmann, E., Lipowski, J., Drela, N., Kowalik, A., Püssa, T., Matt, D., Luik, A., Gozdowski, D., Rembiałkowska, E. (2014). Beetroot (Beta vulgaris L.) and naturally fermented beetroot juices from organic and conventional production: metabolomics, antioxidant levels and anticancer activity. Journal of the Science of Food and Agriculture, 94(3), 2618-2629.
Manach, C., Scalbert, A., Morand, C., Rémésy, C., Jiménez, L. (2004). Polyphenols: food sources and bioavailability. The American Journal of Clinical Nutrition, 79(5), 727-747.
Mattila, P., Hellström, J. (2007). Phenolic acids in potatoes, vegetables, and some of their products. Journal of Food Composition and Analysis, 20(3-4), 152–160.
Marín, L., Miguélez, E.M., Villar, C.J., Lombó, F. (2015). Bioavailability of dietary polyphenols and gut microbiota metabolism: antimicrobial properties. BioMed Research International, 2015, art. no. 905215.
Moon, J.H., Nakata, R., Oshima, S., Inakuma, T., Terao, J. (2000). Accumulation of quercetin conjugates in blood plasma after the short-term ingestion of onion by women. American Journal of Physiology Regulatory, Integrative, Comparative, Physiology, 279(2), R461-R467.
Pandey, K.B., Rizvi, S.I. (2009). Plant polyphenols as dietary antioxidants in human health and disease. Oxidative Medicine and Cellular Longevity, 2(5), 270–278.
Raikos, V., McDonagh, A., Ranawana, V., Duthie, G. (2016). Processed beetroot (Beta vulgaris L.) as a natural antioxidant in mayonnaise: effects on physical stability, texture and sensory attributes. Food Science and Human Wellness, 5(4), 191-198.
Ravichandran, K., Ahmed, A.R., Knorr, D., Smetanska, I. (2012). The effect of different processing methods on phenolic acid content and antioxidant activity of red beet. Food Research International, 48(1), 16-20.
Ravichandran, K., Saw T.M.M.N., Mohdaly A.A.A., Gabr, M.M.A., Kastell, A., Riedel, H., Cai, Z., Knorr, D., Smetanska, I. (2013). Impact of processing of red beet on betalain content and antioxidant activity. Food Research International, 50, 670-675.
Rein, M.J., Renouf, M., Cruz-Hernandez, C., Actis-Goretta, L., Thakkar, S.K., da Silva Pinto, M. (2013). Bioavailability of bioactive food compounds: a challenging journey to bioefficacy. British Journal of Clinical Pharmacology, 75(3), 588–602.
Rembiałkowska, M.E., Hallmann, E., Kazimierczak, R., Skąpska, W., Ardasińska, B. (2011). Ecological methods for fruit and vegetables processing considering health-promoting properties of the finished products. [], (11,2014) (in Polish).
Rodríguez, H., Landete, J.M., Rivas, B. de las, Muñoz, R. (2008). Metabolism of food phenolic acids by Lactobacillus plantarum CECT 748T. Food Chemistry, 107(4), 1393–1398.
Sawicki, T., Bączek, N., Wiczkowski, W. (2016). Betalain profile, content and antioxidant capacity of red beetroot dependent on the genotype and root part. Journal of Functional Foods, 27, 249-261.
Sawicki, T., Topolska, J., Romaszko, E., Wiczkowski, W. (2018). Profile and content of betalains in plasma and urine of volunteers after long-term exposure to fermented red beet juice. Journal of Agricultural and Food Chemistry, 66(16), 4155-4163.
Sawicki, T., Wiczkowski, W. (2018). The effects of boiling and fermentation on betalain profiles and antioxidant capacities of red beetroot products. Food Chemistry, 259, 292-303.
Saxena, M., Saxena, J., Pradhan, A. (2012). Flavonoids and phenolic acids as antioxidants in plants and human health. International Journal of Pharmaceutical Sciences Review and Research, 16(2), 130-134.
Shrestha, A.K., Dahal, N.R., Ndungutse, V. (2010). Bacillus fermentation of soybean: a review. Journal of Food Science and Technology, 6, 1-9.
Svensson, L., Sekwati-Monang, B., Lutz, D.L., Schieber, A., Gänzle, M.G. (2010). Phenolic acids and flavonoids in nonfermented and fermented red sorghum (Sorghum bicolor (L.) Moench). Journal of Agricultural and Food Chemistry, 58(16), 9214–9220.
Székely, D., Szalóki-Dorkó, L., Stéger-Máté, M., Szabó-Nótin, B., Ivanics, J., Monspart-Sényi, J. (2014). Distribution of antioxidant components in roots of different red beets (Beta vulgaris L.) cultivars. Acta Alimentaria, 43, 164–171.
van’t Slot, G., Humpf, H.U. (2009). Degradation and metabolism of catechin, epigallocatechin-3-gallate (EGCG), and related compounds by the intestinal microbiota in the pig cecum model. Journal of Agricultural and Food Chemistry, 57(17), 8041-8048.
Vulić, J.J., Ćebović, T.N., Čanadanović-Brunet, J.M., Ćetković, G.S., Čanadanović, V.M., Djilas, S.M., Tumbas Šaponjac, V.T. (2014). In vivo and in vitro antioxidant effects of beetroot pomace extracts. Journal of Functional Foods, 6, 168–175.
Wiczkowski, W., Piskuła, M.K. (2004). Food flavonoids. Polish Journal of Food and Nutrition Sciences, 13(54), 101-114.
Wiczkowski, W., Romaszko, J., Bucinski, A., Szawara-Nowak, D., Honke, J., Zielinski, H., Piskula, M.K. (2008). Quercetin from shallots (Allium cepa L. var. aggregatum) is more bioavailable than its glucosides. The Journal of Nutrition, 138(5), 885–888.
Wiczkowski, W., Szawara-Nowak, D., Topolska, J. (2015). Changes in the content and composition of anthocyanins in red cabbage and its antioxidant capacity during fermentation, storage and stewing. Food Chemistry, 167, 115-123.
Wiczkowski, W., Szawara-Nowak, D., Sawicki, T., Mitrus, J., Kasprzykowski, Z., Horbowicz, M. (2016). Profile of phenolic acids and antioxidant capacity in organs of common buckwheat sprout. Acta Alimentaria, 45(2), 250-257.
Wiczkowski, W., Topolska, J., Honke, J. (2014). Anthocyanins profile and antioxidant capacity of red cabbages are influenced by genotype and vegetation period. Journal of Functional Foods, 7(1), 201-211.
Wruss, J., Waldenberger, G., Huemer, S., Uygun, P., Lanzerstorfer, P., Müller, U., Höglinger, O., Weghuber, J. (2015). Compositional characteristics of commercial beetroot products and beetroot juice prepared from seven beetroot varieties grown in Upper Austria. Journal of Food Composition and Analysis, 42, 46-55.
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