Synthesis of Galactosyl Mannitol Derivative Using β-Galactosidase from Kluyveromyces lactis
 
More details
Hide details
Publication date: 2017-03-31
 
Pol. J. Food Nutr. Sci. 2017;67(1):33–39
 
KEYWORDS
ABSTRACT
The purpose of the study was to identify the influence of reactive mixture concentration (23-48 g/100 mL), pH (6.5-9.0), presence of NaCl (0.05-0.25 mol/L) and enzyme dose (2850-28,500 LAU/100 g of lactose) on the synthesis of galactosyl mannitol derivative using β-galactosidase from Kluyveromyces lactis. The use of the enzyme dose ranging from 2850 LAU/100 g of lactose to 11,400 LAU/100 g lactose allowed obtaining gal-mannitol at the level of 21.8% total saccharides; higher doses intensified the product decomposition. An increase in the concentration of the reactive mixture had a positive impact on the quantity of gal-mannitol obtained every single time, i.e. 4.39 g were obtained from 100 mL of 23 g/100 mL solution and over 10 g were obtained from a 48 g/100 mL solution. A relatively low increase in product quantity (by ca. 5%) occurred after the pH was increased from 6.5 to 9.0. The use of NaCl rendered better results. An increase in the maximum content of gal-mannitol in the total sugar by 12.8% was observed at the concentration of 0.25 mol/L.
 
CITATIONS (5):
1.
An Ultrahigh-Performance Liquid Chromatography–Time-of-Flight Mass Spectrometry Metabolomic Approach to Studying the Impact of Moderate Red-Wine Consumption on Urinary Metabolome
Adelaida Esteban-Fernández, Clara Ibañez, Carolina Simó, Begoña Bartolomé, M. Moreno-Arribas
Journal of Proteome Research
 
2.
Conventional and non-conventional applications of β-galactosidases
Carlos Vera, Cecilia Guerrero, Carla Aburto, Andrés Cordova, Andrés Illanes
Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics
 
3.
Application of Transgalactosylation Activity of β-Galactosidase from Kluyveromyces lactis for the Synthesis of Ascorbic Acid Galactoside
Aleksandra Wojciechowska, Robert Klewicki, Michał Sójka, Katarzyna Grzelak-Błaszczyk
Applied Biochemistry and Biotechnology
 
4.
Hydrolysis of Lactose and Transglycosylation of Selected Sugar Alcohols by LacA β-Galactosidase from Lactobacillus plantarum WCFS1
Paloma Delgado-Fernandez, Laura Plaza-Vinuesa, Silvia Lizasoain-Sánchez, las de, Rosario Muñoz, María Jimeno, Elisa García-Doyagüez, F. Moreno, Nieves Corzo
Journal of Agricultural and Food Chemistry
 
5.
Active Site Architecture and Reaction Mechanism Determination of Cold Adapted β-d-galactosidase from Arthrobacter sp. 32cB
Maria Rutkiewicz, Anna Bujacz, Marta Wanarska, Anna Wierzbicka-Wos, Hubert Cieslinski
International Journal of Molecular Sciences
 
eISSN:2083-6007
ISSN:1230-0322