THE EFFECT OF SELECTED INDUCTORS ON BIOSYNTHESIS AND PROPERTIES OF β-GALACTOSIDASE.

Two strains of yeast Candida sphaerica WKIIW3b and Kluyveromyces fragilis 28 and hypha fungi Penicillium canescens were grown on culture media where the source of carbon was permeate after ultrafiltration of milk, soybean flour or wheat bran applied individually or in various combinations. In the obtained preparations of b-galactosidase the activity of hydrolysis with ONPG and lactose was determined and the influence of some of the cations and EDTA on the activity of b-galactosidase both with ONPG and with lactose as a substrate was studied. The examination of the properties of transgalactosilation of b-galactosidase preparations synthesised by fungi on media with various sources of carbon involved the application of these preparations for bioconversion of lactose in milk permeate where the concentration of such varied from 17.4 to 28.8%. Saccharides in the obtained products were determined with TLC method.

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Synthesis of Galactosyl Mannitol Derivative Using β-Galactosidase from Kluyveromyces lactis

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eISSN:	2083-6007
ISSN:	1230-0322

W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.