THE EFFECT OF SELECTED INDUCTORS ON BIOSYNTHESIS AND PROPERTIES OF β-GALACTOSIDASE.
 
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Pol. J. Food Nutr. Sci. 2003;53(3):31–37
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ABSTRACT
Two strains of yeast Candida sphaerica WKIIW3b and Kluyveromyces fragilis 28 and hypha fungi Penicillium canescens were grown on culture media where the source of carbon was permeate after ultrafiltration of milk, soybean flour or wheat bran applied individually or in various combinations. In the obtained preparations of b-galactosidase the activity of hydrolysis with ONPG and lactose was determined and the influence of some of the cations and EDTA on the activity of b-galactosidase both with ONPG and with lactose as a substrate was studied. The examination of the properties of transgalactosilation of b-galactosidase preparations synthesised by fungi on media with various sources of carbon involved the application of these preparations for bioconversion of lactose in milk permeate where the concentration of such varied from 17.4 to 28.8%. Saccharides in the obtained products were determined with TLC method.
ISSN:1230-0322