An appropriate level of physical activity is an important element of a strategy for achieving and maintaining the correct body mass, which is a key factor related to reducing health-related risks. This study was aimed at evaluating body mass and body composition as well as selected biochemical indexes in women in relation to their physical activity in the context of health risks. The study included 368 women aged 39 to 60. Depending on the physical activity, significant differences in the body mass, fat and fat free body mass, the BMI and waist and hip circumferences were found. Women with low physical activity had a body mass higher by about 5 kg on average than the active women, and for women with no physical activity the difference amounted to about 9 kg. Women with no physical activity were found to have waist circumferences greater by about 8 cm on average than the active women. The average BMI for active women was significantly lower than for those with no physical activity (25.5 vs. 28.9 kg/m²). The total cholesterol level in the entire group of women included in the study was found to be elevated, regardless of the level of physical activity. The serum levels of glucose, triglycerides and HDL-cholesterol consistent with reference ranges were found in women with no physical activity. The anthropometric parameters in women with low physical activity were likely to increase the risk of developing diet-related diseases.