CHROMATOGRAPHIC SEPARATION OF PHENOLIC COMPOUNDS FROM RAPESEED BY SE-HPLC – A SHORT REPORT

Phenolic compounds from rapeseed were extracted into 80% (v/v) methanol. The crude extract was then separated using size exclusion high-performance liquid chromatography (SE-HPLC) on a TSK G2000SW_XL column with a mobile phase of 45% acetonitrile and 0.1% TFA (v/v). Six peaks were identified on the chromatogram: the main peak originated from sinapin. Calibration of the assay was characterised by an excellent linearity of the sinapin standard (r² = 1.0). The method so described can be used for screening phenolic constituents in rapeseed or canola. It is also useful for the purification of the sinapin as a standard from the crude extract.

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eISSN:	2083-6007
ISSN:	1230-0322

W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.