EFFICIENT IMMOBILIZATION OF MILK CLOTTING ENZYME PRODUCED BY  BACILLUS SPHAERICUS

Milk clotting enzyme produced by Bacillus sphaericus NRC 24 was immobilized efficiently on silica gel with 73% activity retained. Optimum conditions for immobilization of milk clotting enzyme were as follows: pH 5, 4 h contact time and 0.5 mg/mL protein in bulk solution. Optimum pH of the immobilized enzyme was found to be 4 compared to 6 for free enzyme. Optimum temperature ranged from 60 to 70°C for both free and immobilized enzyme preparations. The activation energy (E_a) of the immobilized enzyme was lower than that of the free enzyme (E_a=12.5 and 16.5 Kcal/mol, respectively). Immobilized enzyme showed improved pH, thermal, storage and operational stabilities.

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FUMARIC ACID PRODUCTION BY RHIZPOUS NIGRICANS AND RHIZOPUS ORYZAE USING APPLE JUICE.

CONTINUOUS BIOSYNTHESIS OF AMYLASES BY FREE AND IMMOBILIZED BACILLUS POLYMYXA CELLS

THE EFFECT OF IMMOBILIZATION OF PRIOPONIC ACID BACTERIA IN ALGINATE GEL ON THE COURSE OF FERMENTATION

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eISSN:	2083-6007
ISSN:	1230-0322

W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.