FREE RADICALS AND THEIR ANALYSIS USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY

The hydroperoxyl radical is a much stronger oxidant than superoxide anion-radical and was initially thought to be responsible for the toxic effects of oxygen and cell membrane damage [Liochev & Fridovich, 2001]. HO2· has a relatively long lifetime and so can diffuse to neighbouring structures. At the physiological pH of 7.4 about 1% of superoxide exists in its protonated form. A quantitative method for hydroperoxide determination has been developed by O'Gara et al. [Morisco et al., 2004], based on the oxidation of glutathione (GSH) by hydroperoxide and formation of oxidized glutathione (GSSG). This reaction is catalyzed by glutathione peroxidase. GSH and GSSG were separated on reversed phase column at pH 3 and their concentrations were estimated using electrochemical detection at a glassy carbon electrode. The method was applied for the analysis of picomolar levels of many hydroperoxides in human plasma.

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eISSN:	2083-6007
ISSN:	1230-0322

W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.