IMMUNOREACTIVITY AND SENSITIVITY OF PEPTIDES FROM ß-CASEIN, WHICH INHIBIT LACTOCOCCAL PEPTIDASES, TO HYDROLYSIS BY THERMOLYSIN AND PROTEINASE FROM  PSEUDOMONAS FLUORESCENS

Leszek Stepaniak; Marco Gobbetti

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News New IMPACT FACTOR Volume 70 Authors' Index Volume 70 Reviewers' Index Dietary recommendations during the COVID-19 pandemic. Statement of the Committee of Human Nutrition Science of the Polish Academy of Sciences Our new CiteScore2020 from SCOPUS June 2021 Issue has just been published September 2021 issue has just been released Volume 71's Reviewers Index Our new Impact Factor and Cite Score Our new CiteScore2022 from SCOPUS September 2023 issue has just been published December 2023 issue has just been published March 2024 issue has just been published Volume 73's Reviewers Index June 2024 issue has just been published Our new CiteScore2023 from SCOPUS New IMPACT FACTOR 2023

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IMMUNOREACTIVITY AND SENSITIVITY OF PEPTIDES FROM ß-CASEIN, WHICH INHIBIT LACTOCOCCAL PEPTIDASES, TO HYDROLYSIS BY THERMOLYSIN AND PROTEINASE FROM PSEUDOMONAS FLUORESCENS

Leszek Stepaniak

Marco Gobbetti

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Publication date: 1997-12-31

Pol. J. Food Nutr. Sci. 1997;47(4):41-48

KEYWORDS

β-casein peptides

immunoreactivity

inhibition of hydrolysis

lactococcal peptidases

thermolysin

Pseudomonas fluorescens proteinase

ABSTRACT

Thermolysin and metalloproteinase from Pseudomonas fluorescens P1, both standardized to approximately the same activity on methionine enkephalin, degraded readily bovine ß-casein (ß-CN) fragments 58-70, 58-72, and 1 93-209. These peptides inhibit 70 kDa intracellular endopeptidase and 95 kDa intracellular aminopeptidase from Lactococcus spp. Thedegradation patterns of peptides released from each of the 3 fragments were different for thermolysin and Pseudomonas proteinase. The two enzymes cleaved different peptide bonds of ß-CN f193-209. Cheddar, Norvegia and Roquefort type cheeses contained peptides reactive with antibodies raised against ß-CN f58-70.

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W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.