Search for Author, Title, Keyword
Current issue
In press
Archive
About the Journal
Editorial Policies
Instructions for Authors
2/2018 vol. 68
Stats
Get citation
Rapid and Specific Detection of Escherichia coli O157:H7 in Ground Beef Using Immunomagnetic Separation Combined with Loop-Mediated Isothermal Amplification
Yu Qin
,
 
Santhosh Puthiyakunnon
,
 
Yiduo Zhang
,
 
Xianbo Wu
,
 
Swapna Boddu
,
 
Binde Luo
,
 
Hongying Fan
 
 
 
More details
Hide details
 
Publication date: 2018-06-30
 
 
Pol. J. Food Nutr. Sci. 2018;68(2):115-123
DOI: https://doi.org/10.1515/pjfns-2017-0014
 
 
Article (PDF)
References (48) Citations (6)
 
KEYWORDS
immunomagnetic separation
LAMP
Escherichia coli
EHEC O157:H7
rfbE gene
ground beef
 
ABSTRACT
Escherichia coli O157:H7 is well known for many foodborne outbreaks that lead to fatal infections in human being worldwide. The objective of this study was to develop a rapid and sensitive method for detection of EHEC O157:H7 from ground beef using a method that combined immunomagnetic separation (IMS) with loop-mediated isothermal amplification (LAMP). The EHEC O157:H7 cells were separated with Dynabeads coated with anti-EHEC O157:H7 after a short enrichment for 4 h. Then, EHEC O157:H7 was identified by LAMP assay for amplifying and detecting the rfbE gene, which is highly conserved in all EHEC O157:H7 strains and exhibits strain-specific gene expression. The LAMP method results analyzed with real time turbidity measurements showed a high specificity and sensitivity, with a positive detection rate of amplification of EHEC O157:H7 DNA diluted to a minimum equivalent concentration of 1.8 × 101 CFU/mL, which was 10 times more sensitive than the conventional PCR assay. The IMS followed with LAMP could capture and detect a bacterial concentration as low as 3×101 CFU/mL from the meat samples, which was close to the sensitivity of LAMP assay with pure culture. IMS combined with realistic LAMP method is a simple, rapid, highly specific gene amplification technology that is suitable for implementing as a screening assay in basic laboratory and field test for detecting food contamination.
REFERENCES (48)
1.
Aydin M., Herzig G.P., Jeong K.C., Dunigan S., Shah P., Ahn S., Rapid and sensitive detection of Escherichia coli O157:H7 in milk and ground beef using magnetic bead-based immunoassay coupled with tyramide signal amplification. J. Food Prot., 2014, 77, 100-105.
Google Scholar
 
 
2.
Bapanpally C., Maganty G., Khan S., Kasra A., Morey A., Immunomagnetic separation and visual fluorescence detection of E. coli O157 using AOAC approved SAS Molecular Tests. J. AOAC Int., 2014, 97, 1073-1077.
Google Scholar
 
 
3.
Bell B.P., Griffin P.M., Lozano P., Christie D.L., Kobayashi J.M., Tarr P.I., Predictors of hemolytic uremic syndrome in children during a large outbreak of Escherichia coli O157:H7 infections. Pediatrics, 1997, 100, E12.
Google Scholar
 
 
4.
Bilge S.S., Vary J.C., Jr, Dowell S.F,, Tarr PI., Role of the Escherichia coli O157:H7 O side chain in adherence and analysis of an rfb locus. Infect. Immun., 1996, 64, 4795-4801.
Google Scholar
 
 
5.
Buesa J.B., Collado P., López-Andújar P., Abu-Mallouh R., Rodríguez Díaz J., García Díaz A., Molecular epidemiology of calici viruses causing outbreaks and sporadic cases of acute gastroenteritis in Spain. J. Clin. Microbiol., 2002, 40, 2854-2859．.
Google Scholar
 
 
6.
CDC, Centers for Disease Control and Prevention, Importance of culture confirmation of Shiga toxin-producing Escherichia coli infection as illustrated by outbreaks of gastroenteritis— New York and North Carolina, 2005. MMWR Morb Mortal Wkly Rep., 2006, 55, 1042-1045.
Google Scholar
 
 
7.
Chapman P.A., Ellin M., Ashton R., A comparison of immunomagnetic separation and culture, RevealTM and VIPTM for the detection of E. coli O157 in enrichment cultures of naturally-contaminated raw beef, lamb and mixed meat products. Lett. Appl. Microbiol., 2001, 32, 171-175.
Google Scholar
 
 
8.
Chase-Topping M., Gally D., Low C., Matthews L., Woolhouse M., Super-shedding and the link between human infection and livestock carriage of Escherichia coli O157. Nat. Rev. Microbiol., 2008, 6, 904-912.
Google Scholar
 
 
9.
Desmarchelier P.M., Bilge S.S., Fegan N., Mills L., Vary J.C.Jr., Tarr P.I., A PCR specific for Escherichia coli O157 based on the rfb locus encoding O157 lipopolysaccharide. J. Clin. Microbiol., 1998, 36, 1801-1804.
Google Scholar
 
 
10.
Fan H., Long B., Wu X., Bai Y., Development of a Loop-Mediated Isothermal Amplification Assay for sensitive and rapid detection of Cronobacter sakazakii. Foodborne Pathog. Dis., 2012, 9, 1111-1118.
Google Scholar
 
 
11.
Francois P., Tangomo M., Hibbs J., Bonetti E.J., Boehme C.C., Notomi T., Perkins M.D., Schrenzel J., Robustness of a loop-mediated isothermal amplification reaction for diagnostic applications. FEMS Immunol. Med. Microbiol., 2011, 62, 41–48.
Google Scholar
 
 
12.
Fratamico P.M., Bagi L.K., Cray W.C. Jr., Narang N., Yan X., Medina M., Liu Y., Detection by multiplex real-time polymerase chain reaction assays and isolation of Shiga toxin-producing Escherichia coli serogroups O26, O45, O103, O111, O121, and O145 in ground beef. Foodborne Pathog. Dis., 2011, 8, 601–607.
Google Scholar
 
 
13.
Gianviti A., Rosmini F., Caprioli A., Corona R., Matteucci MC., Principato F., Luzzi I., Rizzoni G., Haemolytic-uraemic syndrome in childhood: surveillance and case-control studies in Italy. Italian HUS Study Group. Pediatr. Nephrol., 1994, 8, 705–9.
Google Scholar
 
 
14.
Gould L.H., Bopp C., Strockbine N., Atkinson R., Baselski V., Body B., Carey R., Crandall C., Hurd S., Kaplan R., Neill M., Shea S., Somsel P., Tobin-D'Angelo M., Griffin P.M., Gerner-Smidt P., Centers for Disease Control and Prevention (CDC), Recommendations for diagnosis of Shiga toxin–producing Escherichia coli infections by clinical laboratories. MMWR Recommend Rep., 2009, 58, 1–14.
Google Scholar
 
 
15.
Han F., Ge B., Quantitative detection of Vibrio vulnificus in raw oysters by real-time loop-mediated isothermal amplification. Int. J. Food Microbiol., 2010, 142, 60–66.
Google Scholar
 
 
16.
Hara-Kudo Y., Nemoto J., Ohtsuka K., Segawa Y., Takatori K., Kojima T., Ikedo M., Sensitive and rapid detection of Vero toxin-producing Escherichia coli using loop-mediated isothermal amplification. J. Med. Microbiol., 2007, 56, 398-406.
Google Scholar
 
 
17.
Hara-Kudo Y., Konishi N., Ohtsuka K., Hiramatsu R, Tanaka H, Konuma HK, Takatori K., Detection of verotoxigenic Escherichia coli O157 and O26 in food by plating methods and LAMP method: A collaborative study. Int. J. Food Microbiol., 2008, 122, 156-161.
Google Scholar
 
 
18.
Hill J., Beriwal S., Chandra I., Paul V.K., Kapil A., Singh T., Wadowsky R.M., Singh V., Goyal A., Jahnukainen T., Johnson J.R., Tarr P.I., Vats A., Loop-mediated isothermal amplification assay for rapid detection of common strains of Escherichia coli. J. Clin. Microbiol., 2008, 46, 2800–2804.
Google Scholar
 
 
19.
Jeníková G., Pazlarová J., Demnerová K., Detection of Salmonella in food samples by the combination of immunomagnetic separation and PCR assay. Int. Microbiol., 2000, 3, 225–229.
Google Scholar
 
 
20.
Jeon S.J., Elzo M., Dilorenzo N., Lamb G.C., Jeong K.C., Evaluation of animal genetic and physiological factors that affect the prevalence of Escherichia coli O157 in cattle. PLoS One, 2013, 8, e55728.
Google Scholar
 
 
21.
Jiang R., Long B., Zeng G., Wang D., Fan H., Wu X., Loop-mediated isothermal amplification for detecting enterohemorrhagic Escherichia coli O157:H7: a comparison with PCR. Nan Fang Yi Ke Da Xue Xue Bao., 2012, 32, 1026-1030.
Google Scholar
 
 
22.
Karch H., Tarr P.I., Bielaszewska M., Enterohaemorrhagic Escherichia coli in human medicine. Int. J. Med. Microbiol., 2005, 295, 405-418.
Google Scholar
 
 
23.
Kou X., Wu Q., Zhang J., Fan H., Development and application of a loop-mediated isothermal amplification assay on rapid and sensitive detection of rotavirus in fecal samples and artificially seeded oysters. Food Contr., 2014, 41, 151-157.
Google Scholar
 
 
24.
Mckee M.L., O’Brien A.D., Investigation of enterohemorrhagic Escherichia coli O157:H7 adherence characteristics and invasion potential reveals a new attachment pattern shared by intestinal E.coli. Infect Immun., 1995, 63, 2070-2074.
Google Scholar
 
 
25.
Mori Y., Nagamine K., Tomita N., Notomi T., Detection of loop-mediated isothermal amplification reaction by turbidity derived from magnesium pyrophosphate formation. Biochem. Biophys. Res. Commun., 2001, 289, 150–154.
Google Scholar
 
 
26.
Mori Y., Notomi T., Loop-mediated isothermal amplification (LAMP): a rapid, accurate, and cost-effective diagnostic method for infectious diseases. J. Infect. Chemother., 2009, 15, 62–69.
Google Scholar
 
 
27.
Nagamine K., Watanage K., Ohtsuka K., Hase T., Notomi T., Loop-mediated isothermal amplification reaction using a nondenatured template. Clin. Chem., 2001, 47, 1742-1743.
Google Scholar
 
 
28.
Nagamine K., Hase T., Notomi T., Accelerated reaction by loop-mediated isothermal amplification using loop primers. Mol. Cell. Probes, 2002, 16, 223–229.
Google Scholar
 
 
29.
Nemoto J., Sugawara C., Akahane K., Hashimoto K., Kojima T., Ikedo M., Konuma H., Hara-Kudo Y., Rapid and specific detection of the thermostable direct hemolysin gene in Vibrio parahaemolyticus by loop-mediated isothermal amplification. J. Food Prot., 2009, 72, 748–754.
Google Scholar
 
 
30.
Notomi T., Okayama H., Masubuchi H., Yonekawa T., Watanabe K., Amino N., Hase T., Loop-mediated isothermal amplification of DNA. Nucleic Acids Res., 2000, 28, E63.
Google Scholar
 
 
31.
Onoue Y., Konuma H., Nakagawa H., Hara-Kudo Y., Fujita T., Kumagai S., Collaborative evaluation of detection methods for Escherichia coli O157:H7 from radish sprouts and ground beef. Int. J. Food Microbiol., 1999, 46, 27-36.
Google Scholar
 
 
32.
Orskov F., Orskov I., Escherichia coli serotyping and disease in man and animals. Can. J. Microbiol., 1992, 38, 699-704.
Google Scholar
 
 
33.
Pruimboom-Brees I.M., Morgan T.W., Ackermann M.R., Nystrom E.D., Samuel J.E., Cornick N.A., Moon H.W., Cattle lack vascular receptors for Escherichia coli O157:H7 Shiga toxins. Proc. Natl. Acad. Sci. USA., 2000, 97, 10325-10329.
Google Scholar
 
 
34.
Ravan H.,, Amandadi M., Sanadgol N., A highly specific and sensitive loop-mediated isothermal amplification method for the detection of Escherichia coli O157:H7. Microb. Pathogen., 2016, 91, 161-165.
Google Scholar
 
 
35.
Saito R., Misawa Y., Moriya K., Koike K., Ubukata K., Okamura N., Development and evaluation of a loop-mediated isothermal amplification assay for rapid detection of Mycoplasma pneumonia. J. Med. Microbiol., 2005, 54, 1037-1041.
Google Scholar
 
 
36.
Sattabongkot J., Tsuboi T., Han E.T., Bantuchai S., Buates S., Loop-mediated isothermal amplification assay for rapid diagnosis of malaria infections in an area of endemicity in Thailand. J. Clin. Microbiol., 2014, 52, 1471–1477.
Google Scholar
 
 
37.
Sen K., Ashbolt N.J., Environmental Microbiology: Current Technology and Water Application. 2011, Caister Academic Press, Norfolk, UK, ISBN; 978(1): 904455-70-7.
Google Scholar
 
 
38.
Sharma V.K., Dean-Nystrom E.A., Detection of enterohemorrhagic Escherichia coli O157:H7 by using a multiplex real-time PCR assay for genes encoding intimin and Shiga toxins. Vet. Microbiol., 2003, 93, 247-260.
Google Scholar
 
 
39.
Taguchi T., Arakaki A., Takeyama H., Haraguchi S., Yoshino M., Kaneko M., Detection of Cryptosporidium parvum oocysts using a microfluidic device equipped with the SUS micromesh and FITC. Biotechnol. Bioeng., 2007, 96, 272-280.
Google Scholar
 
 
40.
Wang F., Jiang L., Ge B., Loop-mediated isothermal amplification assays for detecting shiga toxin-producing Escherichia coli in ground beef and human stools. J. Clin. Microbiol., 2012, 50, 91-97.
Google Scholar
 
 
41.
Wang Y., Wang Y., Luo L., Liu D., Luo X., Xu Y., Hu S., Niu L., Xu J., Ye C., Rapid and sensitive detection of Shigella spp. and Salmonella spp. by Multiple Endonuclease Restriction Real-Time Loop-Mediated Isothermal Amplification Technique. Front. Microbiol., 2015, 6, 1400.
Google Scholar
 
 
42.
Wani S.A., Hussain I., Nabi A., Fayaz I., Nishikawa Y., Variants of eae and stx genes of atypical enteropathogenic Escherichia coli and non-O157 Shiga toxin-producing Escherichia coli from calves. Lett. Appl. Microbiol., 2007, 45, 610-615.
Google Scholar
 
 
43.
Wolf S., Williamson W.M., Hewitt J., Rivera-Aban M., Lin S., Ball A., Scholes P., Greening GE., Sensitive multiplex real—time reverse transcription-PCR assay for the detection of human and animal noroviruses in clinical and environmental samples. Appl. Environ. Microbiol., 2007, 73, 5464—5470.
Google Scholar
 
 
44.
Wright D.J., Chapman P.A., Siddons C.A., Immunomagnetic separation as a sensitive method for isolating E. coli O157 from food samples. Epidemiol. Infect., 1994, 113, 31-39.
Google Scholar
 
 
45.
Xiong Y., Wang P., Lan R., Ye C., Wang H., Ren J., Jing H., Wang Y., Zhou Z., Bai X., Cui Z., Luo X., Zhao A., Wang Y., Zhang S., Sun H., Wang L., Xu J., A novel Escherichia coli O157:H7 clone causing a major hemolytic uremic syndrome outbreak in China. PLoS One, 2012, 7, e36144.
Google Scholar
 
 
46.
Yamazaki W., Ishibashi M., Kawahara R., Inoue K., Development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of Vibrio parahaemolyticus. BMC Microbiol., 2008, 8, 163.
Google Scholar
 
 
47.
Zeng J., Wei H., Zhang L., Liu X., Zhang H., Cheng J., Ma D., Zhang X., Fu P., Liu L., Rapid detection of Vibrio parahaemolyticus in raw oysters using immunomagnetic separation combined with loop-mediated isothermal amplification. Int. J. Food Microbiol., 2014, 174, 123-128.
Google Scholar
 
 
48.
Zhao X., Li Y., Wang L., You L., Xu Z., Li L., He X., Liu Y., Wang J., Yang L., Development and application of a loop-mediated isothermal amplification method on rapid detection Escherichia coli O157 strains from food samples. Mol. Biol. Rep., 2010, 37, 2183-2188.
Google Scholar
 
 
 
CITATIONS (6):
1.
An evaluation of immunomagnetic separation-real-time PCR (IMS-RTiPCR) combined assay for rapid and specific detection of Escherichia coli O157:H7 in raw milk and ground beef
TABAN MERCANOGLU, Sait AYTAC
Food Science and Technology
 
CrossRef
 
 
2.
Immunomagnetic separation: An effective pretreatment technology for isolation and enrichment in food microorganisms detection
Zhouli Wang, Rui Cai, Zhenpeng Gao, Yahong Yuan, Tianli Yue
Comprehensive Reviews in Food Science and Food Safety
 
CrossRef
 
 
3.
Newly isolated Coliphages for Bio-controlling Multidrug Resistant Escherichia coli strains
Mostafa Elbahnasawy, ElSayed ElSayed, Mohamed Azzam
Environmental Nanotechnology, Monitoring & Management
 
CrossRef
 
 
4.
Rapid on-site detection of viable Escherichia coli O157: H7 in lettuce using immunomagnetic separation combined with PMAxx-LAMP and nucleic acid lateral flow strip
Yuanyi Wen, Youjiang Tan, Lichao Zhao, Xinrui Lv, Li Lin, Dezhi Liang, Li Wang
Microchemical Journal
 
CrossRef
 
 
5.
Review in isothermal amplification technology in food microbiological detection
Xiang Li, Xiaolong Zhang, Xiaoli Shi, Hongfei Shi, Zhouping Wang, Chifang Peng
Food Science and Biotechnology
 
CrossRef
 
 
6.
Establishment of real-time fluorescence and visual LAMP for rapid detection of Escherichia coli O157:H7 and kits construction
Zuwei Wang, Xinping Cui, Antuo Hu, Zhaoxin Lu, Fanqiang Meng, Libang Zhou, Xiaomei Bie
Letters in Applied Microbiology
 
CrossRef
 
 
Share
Send by email
RELATED ARTICLE
EFFECT OF XANTHAN PROTECTIVE COATING WITH LACTOBACILLUS SAKEI CULTURE ADDITION ON THE MICROBIOLOGICAL SAFETY AND THE QUALITY OF PORK STORED UNDER REFRIGERATION
THE ISOLATION AND DETECTION OF LISTERIA FROM ARTIFICIALLY CONTAMINATED FOOD BY USE OF IMMUNOMAGNETIC SEPARATION AND CULTIVATION PROCEDURES.
VIABILITY LOSS OF SOME FOOD-BORNE PATHOGENS IN MILK FERMENTED WITH LACTOBACILLUS ACIDOPHILUS.
Indexes
 
Keywords index
Topics index
Authors index
eISSN:2083-6007
ISSN:1230-0322
Journals System - logo
W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.
© 2006-2024 Journal hosting platform by Bentus
Scroll to top