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ORIGINAL ARTICLE
Tumor Anti-Initiation and Anti-Progression Properties of Sulphated-Extract of Colocasia esculenta
1
Clinical Laboratory Sciences Department, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
2
Department of Natural and Microbial Products Chemistry, National Research Centre, Dokki, Cairo 12622, Egypt
3
Cancer Biology and Genetics Laboratory, Centre of Excellence for Advanced Sciences, National Research Centre, 33 El Buhouth St. Dokki, Cairo, 12622, Egypt
4
Biochemistry Department, National Research Centre, 33 El Buhouth St. Dokki, Cairo, 12622, Egypt
5
Radiological Sciences Department, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia
Submission date: 2021-05-25
Final revision date: 2021-10-01
Acceptance date: 2021-10-04
Online publication date: 2021-10-26
Publication date: 2021-10-26
Corresponding author
Amira M. Gamal-Eldeen
Clinical Laboratory Sciences Department, College of Applied Medical Sciences, Taif University, Saudi Arabia
Clinical Laboratory Sciences Department, College of Applied Medical Sciences, Taif University, Saudi Arabia
Pol. J. Food Nutr. Sci. 2021;71(4):393-401
KEYWORDS
Colocasia esculentaCYP1Acancer chemoprevention & tumor anti-initiatingHDACmacrophage functionbreast MCF-7 carcinoma
TOPICS
ABSTRACT
Colocasia esculenta (Taro) is an edible tuberous plant; however, corms are its most worldwide consumed part while the corm powder is widely used in food industries. In this work, a sulphated polysaccharide extract of C. esculenta corm (SCE) was prepared and its cancer chemopreventive properties were explored. The amending of carcinogen metabolism and radical scavenging affinity revealed that SCE is a strong tumor anti-initiation agent via suppressing cytochrome P450-1A and enhancing glutathione and the carcinogen detoxification enzyme; glutathione S-transferase. SCE exhibited a strong scavenging affinity towards critical radicals (hydroxyl and peroxyl). It induced lymphocyte growth and modulated the macrophage functions into an anti-inflammatory profile, via elevating macrophage proliferation and its binding affinity of fluorescein isothiocyanate-lipopolysaccharide (FITC-LPS) and inhibiting nitric oxide and tumor necrosis factor-α generation. Furthermore, SCE showed a potent cytotoxicity against human breast MCF-7 carcinoma cells (IC50 27.73 µg/mL), whereas SCE treatment inhibited the activity of histone deacetylase (HDAC IC50 37.70 µg/mL) and disturbed the pattern of cell cycle phases. An arrest in both S- and G2/M-phases was linked with shifted cell populations towards late apoptosis and necrosis, as detected by flow cytometry. SCE is a promising cancer chemopreventive agent to be used in healthy food industries and for the high breast cancer-risk population.
ACKNOWLEDGEMENTS
The authors gratefully acknowledge the support of the Deanship of Scientific Research, Taif University.
FUNDING
This work was supported by Taif University Researchers Supporting Project Number (TURSP-2020/103).
REFERENCES (50)
1.
Aggarwal, B.B., Shishodia, S. (2006). Molecular targets of dietary agents for prevention and therapy of cancer. Biochemical Pharmacology, 71(10),1397–1421. https://doi.org/10.1016/j.bcp.... .
2.
Bertram, J.S. (2000). The molecular biology of cancer. Molecular Aspects of Medicine, 21(6), 167–223. https://doi.org/10.1016/S0098-....
3.
Bose, D.S., Sudharshan, M., Chavhan, S.W. (2005). New protocol for Biginelli reaction – a practical synthesis of monastrol. Arkivoc, iii, 228–236.
4.
Cao, G., Prior, R.L. (1999). Measurement of oxygen radical absorbance capacity in biological samples. Methods in Enzymology, 299, 50–62. https://doi.org/10.1016/s0076-....
5.
Carracedo, J., Ramirez, R., Martin-Malo, A., Rodriguez, M., Aljama, P. (2002). The effect of LPS, uraemia, and haemodialysis membrane exposure on CD14 expression in mononuclear cells and its relation to apoptosis. Nephrology Dialysis Transplantation, 17(3), 428–434. https://doi.org/10.1093/ndt/17....
6.
Crespi, C.L., Miller, V.P., Penman, B.W. (1997). Microtiter plate assays for inhibition of human, drug-metabolizing cytochromes P450. Analytical Biochemistry, 248(1), 188–190. https://doi.org/10.1006/abio.1....
7.
DuBois, M., Gilles, K.A., Hamilton, J.K., Rebers, P.A., Smith, F. (1956). Colourimetric method for determination of sugars and related substances. Analytical Chemistry, 28(3), 350–356. https://doi.org/10.1021/ac6011....
8.
Eleazu, C.O., Iroaganachi, M., Eleazu, K.C. (2013). Ameliorative potentials of cocoyam (Colocasia esculenta L). and unripe plantain (Musa paradisiaca L). on the relative tissue weights of streptozotocin‐induced diabetic rats. Journal of Diabetes Research, 2013, art. no. 160964. https://doi.org/10.1155/2013/1....
9.
Ferreres, F., Gonçalves, R.F., Gil-Izquierdo, A., Valentão, P., Silva, A.M.S., Silva, J.B. (2012). Further knowledge on the phenolic profile of Colocasia esculenta (L). Schott. Journal of Agricultural and Food Chemistry, 60(28), 7005-7015. https://doi.org/10.1021/jf3017....
10.
Gamal-Eldeen, A.M., Amer, H., Alrehaili, A.A., Saleh, A., Al Ghamdi, A, Hawsawi, N.M., Salman, A., Raafat, B. (2021). Cancer chemopreventive properties of sulfated Enterolobium cyclocarpum extract. Nutrition and Cancer, 73, 856-868. https://doi.org/10.1080/016355....
11.
Gamal-Eldeen, A.M., Ahmed, E.F., Abo-Zeid, M.A. (2009). In vitro cancer chemopreventive properties of polysaccharide extract from the brown alga, Sargassum latifolium. Food and Chemical Toxicology, 47(6), 1378-1384. https://doi.org/10.1016/j.fct.....
12.
Gamal-Eldeen, A.M., Amer, H., Helmy, W.A. (2006). Cancer chemopreventive and anti-inflammatory activities of chemically modified guar gum. Chemico-Biological Interactions, 161(3), 229–240. https://doi.org/10.1016/j.cbi.....
13.
Gamal-Eldeen, A.M., Amer, H., Helmy, W.A., Ragab, H.M., Talaat, R.M. (2007a). Antiproliferative and cancer-chemopreventive properties of sulfated glycosylated extract derived from Leucaena leucocephala. Indian Journal of Pharmaceutical Sciences, 69(6), 805–811. https://doi.org/10.4103/0250-4....
14.
Gamal-Eldeen, A.M., Amer, H., Helmy, W.A., Talaat, R.M., Ragab, H. (2007b). Chemically-modified polysaccharide extract derived from Leucaena leucocephala alters RAW 2647 murine macrophage functions. International Immunopharmacology, 7(6), 871–878. https://doi.org/10.1016/j.inti... .
15.
Gamal-Eldeen, A.M., Kawashty, S., Ibrahim, L., Shabana, M., El-Negoumy, S. (2004). Evaluation of antioxidant, anti-inflammatory, and antinociceptive properties of aerial parts of Vicia sativa and its flavonoids. Journal of Natural Remedies, 4, 81–96. https://doi.org/10.18311/JNR/2... .
16.
Gerhäuser, C., Klimo, K., Heiss, E., Neumann, I., Gamal-Eldeen, A., Knauft, J., Liu, G.Y., Sitthimonchai, S., Frank, N. (2003). Mechanism-based in vitro screening of potential cancer chemopreventive agents. Mutation Research, 523–524, 163–172. https://doi.org/10.1016/s0027-....
17.
Gonçalves, R.F., Silva, A.M., Silva, A.M., Valentão, P., Ferreres, F., Gil-Izquierdo, A., Silva, J.B., Santos, D., Andrade, P.B. (2013). Influence of taro (Colocasia esculenta L Schott). growth conditions on the phenolic composition and biological properties. Food Chemistry, 141(4), 3480–3485. https://doi.org/10.1016/j.food....
18.
Griffith, O.W. (1980). Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Analytical Biochemistry, 106(1), 207-212. https://doi.org/10.1016/0003-2....
19.
Habig, W.H., Pabst, M.J., Jakoby, W.B. (1974). Glutathione S-transferases: The first enzymatic step in mercapturic acid formation. Journal of Biological Chemistry, 249(22), 7130–7139. https://doi.org/10.1016/S0021-....
20.
Hansen, M.B., Nielsen, S.E., Berg, K. (1989). Re-examination and further development of a precise and rapid dye method for measuring cell growth/cell kill. Journal of Immunological Methods, 119(2), 203–210. https://doi.org/10.1016/0022-1....
21.
Hayes, J.D., Dinkova-Kostova, A.T., McMahon, M. (2009). Cross-talk between transcription factors AhR and Nrf2, lessons for cancer chemoprevention from dioxin. Toxicological Sciences, 111(2), 199-201. https://doi.org/10.1093/toxsci....
22.
Hunt, J. (1980). Determination of total sulfur in small amounts of plant material. The Analyst, 105(1246), 83-85. https://doi.org/10.1039/an9800....
23.
Kaushal, P., Kumar, V., Sharma, H.K. (2015). Utilization of taro (Colocasia esculenta). A review. Journal of Food Science and Technology, 52, 27–40. https://doi.org/10.1007/s13197....
24.
Keyaerts, E., Vijgen, L., Pannecouque, C., Van Damme, E., Peumans, W., Egberink, H., Balzarini, J., Van Ranst, M. (2007). Plant lectins are potent inhibitors of coronaviruses by interfering with two targets in the viral replication cycle. Antiviral Research, 75(3), 179-187. https://doi.org/10.1016/j.anti....
25.
Kitchens, R.L. (2000). Role of CD14 in cellular recognition of bacterial lipopolysaccharides. Chemical Immunology, 74, 61–67. https://doi.org/10.1159/000058....
26.
Kundu, N., Campbell, P., Hampton, B., Lin, C.Y., Ma, X., Ambulos, N., Zhao, X.F., Goloubeva, O., Holt, D., Fulton, A.M. (2012). Antimetastatic activity isolated from Colocasia esculenta (taro). Anticancer Drugs, 23(2), 200-211. https://doi.org/10.1097/CAD.0b....
27.
Larsen, B., Haug, A., Painter, J.T.J. (1966). Sulfated polysaccharides in brown algae. I. Isolation and preliminary characterization of three sulfated polysaccharides from Ascophylum nodosum. Acta Chemica Scandinavica, 20, 219–230. https://doi.org/10.3891/acta.c....
28.
Lee, S., Wee, W., Yong, J., Syamsumir, D. (2011). Antimicrobial, antioxidant, anticancer property and chemical composition of different parts (corm, stem and leaves) of Colocasia esculenta extract. Annales Universitatis Mariae Curie‐Sklodowska, Lublin – Polonia, Sectio DDD, Pharmacia, 24(3), 9–16.
29.
Li, Y., Seto, E. (2016). HDACs and HDAC inhibitors in cancer, development and therapy. Cold Spring Harbor Perspectives in Medicine, 6(10), art. no. a026831. https://doi.org/10.1101/cshper....
30.
Lim, T.K. (2015). Colocasia esculenta in Edible Medicinal and Non-Medicinal Plants. Springer, Germany. Vol 9, pp. 454– 492. https://doi.org/10.1007/978-94....
31.
Lowry, O.H., Rosenbrough, N.J., Farr, A.L., Randall, R.J. (1951). Protein measurement with the Folin–phenol reagent. Journal of Biological Chemistry, 193(1), 265–275. https://doi.org/10.1016/S0021-....
32.
Lund, E. (2011). An exposure driven functional model of carcinogenesis. Medical Hypotheses, 77(2), 195-198. https://doi.org/10.1016/j.mehy....
33.
Mähner, C., Lechner, M.D., Nordmeier, E. (2001). Synthesis and characterization of dextran and pullulan sulfate. Carbohydrate Research, 331(2), 203–208. https://doi.org/10.1016/s0008-....
34.
Mescher, M., Haarmann-Stemmann, T. (2018). Modulation of CYP1A1 metabolism, from adverse health effects to chemoprevention and therapeutic options. Pharmacology, Therapeutics, 187, 71-87. https://doi.org/10.1016/j.phar....
35.
Mollakhalili, M.N., Mortazavian, A.M., Bahadori, M.A., Sohrabvandi, S., Aghaei, M.F. (2017). Phytochemicals in cancer prevention, a review of the evidence. International Journal of Cancer Management, 10(1), art. no. e7219. https://doi.org/10.17795/ijcp-... .
36.
Moorcroft, M., Davis, J., Compton, R.G. (2001). Detection and determination of nitrate and nitrite, a review. Talanta, 54(5), 785–803. https://doi.org/10.1016/s0039-....
37.
Noorfarahzilah, M., Lee, J.S., Sharifudin, M., MohdFadzelly, A., Hasmadi, M. (2014). Applications of composite flour in development of food products. International Food Research Journal, 21(6), 2061– 2074.
38.
Nwauzoma, A., Dappa, M.S. (2013). Ethnobotanical studies of Port Harcourt Metropolis, Nigeria. ISRN Botany, 2013, art. no. 829424. https://doi.org/10.1155/2013/8... .
39.
Park, H.R., Lee, H.S., Cho, S.Y., Kim, Y.S., Shin, K.S. (2013). Antimetastatic effect of polysaccharide isolated from Colocasia esculenta is exerted through immunostimulation. International Journal of Molecular Medicine, 31(2), 361-368. https://doi.org/10.3892/ijmm.2....
40.
Partridge, S.M. (1949). Aniline hydrogen phthalate as spraying reagent for chromatography of sugars. Nature, 164, 443–446. https://doi.org/10.1038/164443....
41.
Pereira, P.R., Corrêa, A., Vericimo, M.A., Paschoalin, V. (2018). Tarin, a potential immunomodulator and COX-inhibitor lectin found in taro (Colocasia esculenta). Comprehensive Reviews in Food Science and Food Safety, 17(4), 878-891. https://doi.org/10.1111/1541-4....
42.
Prabhu, V., Guruvayoorappan, C. (2010). Nitric oxide, pros and cons in tumor progression. Immunopharmacology and Immunotoxicology, 32(3), 387–392. https://doi.org/10.3109/089239....
43.
Reyad‐ul‐Ferdous, M., Arman, M.S.I., Tanvir, M.M.I., Sumi, S., Siddique, K.M.M.R., Billah, M.M., Islam, S. (2015). Biologically potential for pharmacologicals and phytochemicals of medicinal plants of Colocasia esculenta, A comprehensive review. American Journal of Clinical and Experimental Medicine, 3(5-1), 7–11. https://doi.org/10.11648/j.ajc... .
44.
Sakano, Y., Mutsuga, M., Tanaka, R., Suganuma, H., Inakuma, T., Toyoda, M. (2005). Inhibition of human lanosterol synthase by the constituents of Colocasia esculenta (taro). Biological and Pharmaceutical Bulletin, 28(2), 299– 304. https://doi.org/10.1248/BPB.28....
45.
Sun, S.Y., Hail, N. J.R., Lotan, R. (2004). Apoptosis as a novel target for cancer chemoprevention. Journal of the National Cancer Institute, 96(5), 662–672. https://doi.org/10.1093/jnci/d....
46.
Tan, A.C., Konczak, I., Sze, D.M., Ramzan, I. (2011). Molecular pathways for cancer chemoprevention by dietary phytochemicals. Nutrition and Cancer, 63(4), 495-505. https://doi.org/10.1080/016355....
47.
Tanaka, T., Ishigamori, R. (2011). Understanding carcinogenesis for fighting oral cancer. Journal of Oncology, 2011, art. no. 603740. https://doi.org/10.1155/2011/6....
48.
Temesgen, M., Retta, N. (2015). Nutritional potential, health and food security benefits of taro Colocasia esculenta (L). A review. Food Science and Quality Management, 36, 23–30.
49.
Xu, X., Lai, Y., Hua, Z.-C. (2019). Apoptosis and apoptotic body, disease message and therapeutic target potentials. Bioscience Reports, 39(1), art. no. BSR20180992. https://doi.org/10.1042/BSR201....
50.
Yang, J., Du, Y., Wen, Y., Li, T., Hu, L. (2003). Sulfation of Chinese lacquer polysaccharides in different solvents. Carbohydrate Polymers, 52(4), 397-403. https://doi.org/10.1016/S0144-....
CITATIONS (1):
1.
Preparation, structural characteristics, and application of taro polysaccharides in food
Wenting Zhang, Gangliang Huang
Journal of the Science of Food and Agriculture
Wenting Zhang, Gangliang Huang
Journal of the Science of Food and Agriculture
| eISSN: | 2083-6007 |
| ISSN: | 1230-0322 |
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