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ORIGINAL ARTICLE
A Step Forward Towards Exploring Nutritional and Biological Potential of Mushrooms: A Case Study of Calocybe gambosa (Fr.) Donk Wild Growing in Serbia
Jovana Dragan Petrovic 1  
,   Ângela Fernandes 2  
,   Dejan Stojković 1  
,   Marina Soković 1  
,   Lillian Barros 2  
,   Isabel C.F.R. Ferreira 2  
,   Aditya Shekhar 3  
,   Jasmina Glamočlija 1  
 
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1
Plant Physiology, Institute for Biological Research Sinisa Stankovic, University of Belgrade, National Institute of Republic of Serbia, Bulevar despota Stefana 142, 11000 Belgrade, Serbia
2
Centro de Investigação de Montanha (CIMO), Instituto Politécnico de Bragança, Campus de Santa Apolónia, 5300-253, Bragança, Portugal
3
Helmholtz Centre for Infection Research, Inhoffenstraße 7, 38124 Braunschweig, Germany
CORRESPONDING AUTHOR
Jovana Dragan Petrovic   

Plant Physiology, Institute for Biological Research Sinisa Stankovic, University of Belgrade, National Institute of Republic of Serbia, Bulevar despota Stefana 142, 11060, Belgrade, Serbia
Submission date: 2021-09-27
Final revision date: 2021-12-08
Acceptance date: 2021-12-13
 
 
KEYWORDS
TOPICS
ABSTRACT
Edible mushrooms have been appreciated globally for their organoleptic, nutritional and chemical properties. In the present study, fruiting body of Calocybe gambosa, wild growing in Serbia, has been chemically characterized (content of macronutrients, soluble sugars, tocopherols, fatty and organic acids) and its bioactive (antimicrobial and antioxidant) properties were evaluated. The results obtained suggest that this mushroom is a source of carbohydrates and proteins (72 g/100 g dry weight (dw) and 16 g/100 g dw, respectively), with a low fat content (1.5 g/100 g dw). Sugar analysis revealed the presence of trehalose (3.82 g/100 g dw) and mannitol (0.22 g/100 g dw). Tocopherol composition revealed the presence of α-tocopherol (7.8 μg/100 g dw), while fatty acid analysis revealed the presence of 24 fatty acids with the prevalence of polyunsaturated fatty acids. Amongst organic acids, oxalic, quinic, malic, citric and fumaric acid were detected (2.8 g/100 g dw, 1.45 g/100 g dw, 11.3 g/100 g dw, 1.3 g/100 g dw, 0.08 g/100 g dw, respectively). Comprehensive antioxidant activity analysis (reducing power, DPPH radical scavenging activity, β-carotene/linoleate and TBARS assays) indicate that the mushroom is a perspective antioxidant source, whereas its antimicrobial potential turned out to be moderate. Nevertheless, at a sub-inhibitory level, the methanolic extract disrupted cell-to-cell communication using Pseudomonas aeruginosa PAO1 as a model system. Finally, enrichment of oatmeal cookies with C. gambosa flakes not only improved their nutritional value, but was praised among the participants in sensory evaluation test, indicating that along with results of chemical composition and biological activity, this mushroom has a potential to be regarded as functional food.
FUNDING
This research was funded by the Ministry of Education, Science and Technological Development of the Republic of Serbia, grant number 451-03-9/2021-14/ 200007. The authors acknowledge the Foundation for Science and Technology (FCT, Portugal) for financial support by national funds FCT/MCTES to CIMO (UIDB/00690/2020).
 
REFERENCES (38)
1.
Alves, M., Ferreira, I.F.R., Dias, J., Teixeira, V., Martins, A. Pintado, M. (2012). A review on antimicrobial activity of mushroom (Basidiomycetes) extracts and isolated compounds. Planta Medica, 78, 1707–1718. https://doi.org/10.1055/s-0032....
 
2.
Alves, M.J., Ferreira, I.C.F.R., Froufe, H.J.C., Abreu, R.M.V, Martins, A., Pintado, M. (2013). Antimicrobial activity of phenolic compounds identified in wild mushrooms, SAR analysis and docking studies. Journal of Applied Microbiology, 115, 346–357. https://doi.org/10.1111/jam.12....
 
3.
Angelini, P., Tirillini, B., Venanzoni, R., (2012). In vitro antifungal activity of Calocybe gambosa extracts against yeasts and filamentous fungi. African Journal of Microbiology Research, 6, 1810-1814. https://doi.org/10.5897/AJMR11....
 
4.
AOAC (2016). Official Methods of Analysis of AOAC International. Official Methods of Analysis of AOAC International; AOAC International: Gaithersburg, MD, USA.
 
5.
Barroetaveña, C., Toledo, C.V. (2017). The nutritional benefits of mushrooms. Chapter 3, In: I.C.F.R. Ferreira, P. Morales, L. Barros (Eds)., Wild Plants, Mushroom and Nuts. Functional Food Properties and Applications. Wiley Blackwell, pp. 65-81. https://doi.org/10.1002/978111....
 
6.
Barros, L., Calhelha, R.C., Vaz, J.A., Ferreira, I.C.F.R., Baptista, P., Estevinho, L.M. (2007). Antimicrobial activity and bioactive compounds of Portuguese wild edible mushrooms methanolic extracts. European Food Research and Technology, 225, 151–156. https://doi.org/10.1007/s00217....
 
7.
Barros, L., Pereira, C., Ferreira, I.C.F.R. (2013). Optimized analysis of organic acids in edible mushrooms from Portugal by ultrafast liquid chromatography and photodiode array detection. Food Analytical Methods, 6, 309-316. https://doi.org/10.1007/s12161....
 
8.
Beluhan, S., Ranogajec, A. (2011). Chemical composition and non-volatile components of Croatian wild edible mushrooms. Food Chemistry, 124, 1076-1082. https://doi.org/10.1016/j.food....
 
9.
Carocho, M., Ferreira, I.C.F.R. (2013). A review on antioxidants, prooxidants and related controversy: Natural and synthetic compounds, screening and analysis methodologies and future perspectives. Food & Chemical Toxicology, 51, 15-25. https://doi.org/10.1016/j.fct.....
 
10.
CLSI (2009). Clinical and Laboratory Standards Institute Methods for dilution antimicrobial susceptibility tests for bacteria that grow aerobically. In: Approved standard, 8th ed. CLSI publication M07-A8. Clinical and Laboratory Standards Institute, Wayne, PA, USA.
 
11.
El Fouly, M.Z., Sharaf, A.M., Shahin, A.A.M., El-Bialy, H.A., Omara, A.M.A. (2015). Biosynthesis of pyocyanin pigment by Pseudomonas aeruginosa. Journal of Radiation Research and Applied Sciences, 8, 36–48. https://doi.org/10.1016/j.jrra....
 
12.
Ferreira, I.C.F.R., Barros, L., Abreu, R. (2009). Antioxidants in wild mushrooms. Current Medicinal Chemistry, 16(12), 1543-1560. https://doi.org/10.2174/092986....
 
13.
Heleno, S., Barros, L., Sousa, M., Martins, A., Ferreira, I.C.F.R. (2010). Tocopherols composition of Portuguese wild mushrooms with antioxidant capacity. Food Chemistry, 119(4), 1443-1450. https://doi.org/10.1016/j.food....
 
14.
Li, M., Leilei, Y., Zhao, J., Zhang, H., Chen, W., Zhai, Q., Fengwei, T. (2021). Role of dietary edible mushrooms in the modulation of gut microbiota. Journal of Functional Foods, 83, art. no. 104538. https://doi.org/10.1016/j.jff.....
 
15.
Ma, G.X., Du, H.J., Hu, Q.H., Yang, W.J., Pei, F., Xiao, H. (2021). Health benefits of edible mushroom polysaccharides and associated gut microbiota regulation. Critical Reviews in Food Science and Nutrition, https://doi.org/10.1080/104083....
 
16.
Mithul Aravind, S., Wichienchot, S., Tsao, S., Ramakrishnan, S., Chakkaravarthi, S. (2021). Role of dietary polyphenols on gut microbiota, their metabolites and health benefits. Food Research International, 142, art. no. 110189. https://doi.org/10.1016/j.food....
 
17.
Mridu, C., Atri, N.S. (2017). Nutritional and nutraceutical characterization of three wild edible mushrooms from Haryana, India. Mycosphere, 8(8), 1035-1043. https://doi.org/10.5943/mycosp....
 
18.
Petrović, J., Glamoclija, J., Stojković, D., Ciric, A., Barros, L., Ferreira, I., Soković, M. (2015). Nutritional value, chemical composition, antioxidant activity and enrichment of cream cheese with chestnut mushroom Agrocybe aegerita (Brig.) Sing. Journal of Food Science and Technology Mysore, 52, 6711–6718. https://doi.org/10.1007/s13197....
 
19.
Petrović, J., Glamočlija, J., Ilić-Tomić, T., Soković, M., Robajac, D., Nedić, O., Pavić, A. (2020). Lectin from Laetiporus sulphureus effectively inhibits angiogenesis and tumor development in the zebrafish xenograft models of colorectal carcinoma and melanoma. International Journal of Biological Macromolecules, 148, 129-139, https://doi.org/10.1016/j.ijbi....
 
20.
Rathee, S., Rathee, D., Rathee, D., Kumar, V., Rathee, P. (2012). Mushrooms as therapeutic agents. Revista Brasileira de Farmacognosia, 22, 459–474. https://doi.org/10.1590/S0102-....
 
21.
Reis, F.S., Barros, L., Martins, A., Ferreira, I.C.F.R. (2012a). Chemical composition and nutritional value of the most widely appreciated mushrooms: an inter-species comparative study. Food & Chemical Toxicology, 50, 191-197. https://doi.org/10.1016/j.fct.....
 
22.
Reis, F.S., Martins, A., Barros, L., Ferreira, I.C.F.R. (2012b). Antioxidant properties and phenolics profile of the most widely appreciated cultivated mushrooms: a comparative study between in vivo and in vitro samples. Food & Chemical Toxicology, 50, 1201-1207. https://doi.org/10.1016/j.fct.....
 
23.
Ribeiro, B., Guedes de Pinho, P., Andrade, P.B., Baptista, P., Valentão, P. (2009). Fatty acid composition of wild edible mushrooms species: A comparative study. Microchemical Journal, 93, 29–35. https://doi.org/10.1016/j.micr....
 
24.
Sang, H., Xie, Y., Su, X., Zhang, M., Zhang, Y., Liu, K., Wang, J. (2020). Mushroom Bulgaria inquinans modulates host immunological response and gut microbiota in mice. Frontiers in Nutrition, 7, art. no. 144. https://doi.org/10.3389/fnut.2....
 
25.
Soković, M., Ćirić, A., Glamočlija, J., Nikolić, M., Griensven, L.J.L.D. (2014). Agaricus blazei hot water extract shows anti quorum sensing activity in the nosocomial human pathogen Pseudomonas aeruginosa. Molecules, 19(4), 4189-4199. https://doi.org/10.3390/molecu....
 
26.
Soković, M., Ćirić, A., Glamočlija, J., Stojković, D. (2017). The bioactive properties of mushrooms. Chapter 4, In: I.C.F.R. Ferreira, P. Morales, L. Barros (Eds)., Wild Plants, Mushroom and Nuts. Functional Food Properties and Applications. Wiley Blackwell, pp. 83-122. https://doi.org/10.1002/978111....
 
27.
Soobrattee, M.A., Neergheen, V.S., Luximon-Ramma, A., Aruoma, O.I., Bahorun, T. (2005). Phenolics as potential antioxidant therapeutic agents: mechanism and actions. Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis, 579(1-2), 200–213. https://doi.org/10.1016/j.mrfm....
 
28.
Stojković, D., Reis, F., Glamoclija, J., Ciric, A., Barros, L., Van Griensven, L., Ferreira, I., Soković, M. (2014). Cultivated strains of Agaricus bisporus and A. brasiliensis: Chemical characterization and evaluation of antioxidant and antimicrobial properties for the final healthy product-natural preservatives in yoghurt. Food & Function, 5, 1602-1612. https://doi.org/10.1039/c4fo00....
 
29.
Su, C.H., Laib, M.N., Ng, L.T. (2013). Inhibitory effects of medicinal mushrooms on α-amylase and α-glucosidase – enzymes related to hyperglycemia. Food & Function, 4, 644-649. https://doi.org/10.1039/c3fo30....
 
30.
Valverde, M.E., Hernández-Pérez, T., Paredes-López, O. (2015). Edible mushrooms: improving human health and promoting quality life. International Journal of Microbiology, 2015, art. no. 376387. https://doi.org/10.1155/2015/3....
 
31.
Vamanu, E., Gatea, F. (2020). Correlations between microbiota bioactivity and bioavailability of functional vompounds: A mini-teview. Biomedicines, 8(2), art. no. 39. https://doi.org/10.3390/biomed....
 
32.
Vaz, A.J., Heleno, S.A., Martins, A., Almeida, M.G., Vasconcelos, M.H., Ferreira, I.C.F.R. (2010). Wild mushrooms Clitocybe alexandri and Lepista inversa: In vitro tumor cell lines. Food & Chemical Toxicology, 48(10), 2881-2884. https://doi.org/10.1016/j.fct.....
 
33.
Vaz, J., Barros, L., Martins, A., Santos-Buelga, C., Vasconcelos, H.M., Ferreira, I.C.F.R. (2011). Chemical composition of wild edible mushrooms and antioxidant properties of their water soluble polysaccharidic and ethanolic fractions. Food Chemistry, 126(2), 610-616. https://doi.org/10.1016/j.food....
 
34.
Wasser, S. (2014). Medicinal mushroom science: Current perspectives, advances, evidences, and challenges. Biomedicinal Journal, 37(6), 345–356. https://doi.org/10.4103/2319-4....
 
35.
Wilson, J.W., Schurr, M.J., LeBlanc, C.L., Ramamurthy, R., Buchanan, K.L., Nickerson, C.A. (2002). Mechanisms of bacterial pathogenicity. Postgraduate Medical Journal, 78, 216–224. https://doi.org/10.1136/pmj.78....
 
36.
Yeo, S.S.M., Tham, F.Y. (2012). Anti-quorum sensing and antimicrobial activities of some traditional Chinese medicinal plants commonly used in South-East Asia. Malaysian Journal of Microbiology, 8, 11-20. https://doi.org/10.21161/mjm.3....
 
37.
Zhong, J.H.X. (2009). Secondary metabolites from higher fungi: Discovery, bioactivity and bioproduction. Advances in Biochemical Engineering/Biotechnology, 113, 79–150. https://doi.org/10.1007/10_200....
 
38.
Zhu, H, He, C.C., Chu, Q.H., (2011). Inhibition of quorum-sensing in Chromobacterium violaceum by pigments from Auricularia auricular. Letters in Applied Microbiology, 52, 269-274. https://doi.org/10.1111/j.1472....
 
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