ANTIBACTERIAL ACTIVITY OF DIFFERENT FRACTIONS FROM THE CULTURE OF PROPIONIBACTERIUM ACIDIPROPIONICI
 
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Pol. J. Food Nutr. Sci. 1999;49(1):23–29
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ABSTRACT
The effect of medium and time of culturing of Propionibacterium acidipropionici was studied on the biosynthesis of metabolites of antibacterial activity against selected gram-negative Enterobacteriaceae rods and Staphylococcus aureus. The strain was found to synthesise antibacterial metabolites others than volatile acids exclusively when cultured in natural media, i.e. milk and whey of pH being adjusted every 24 h to 6.8 at optimal temperature. The antibacterial activity of neutralised cultures was observed after 5 days of incubation. Heating of the culture at 550C for 5 min caused that the antibacterial activity disappeared. Fractionation of the culture by separating the biomass by centrifugation and filtering the supernatant through bacteriological filter showed that biomass suspended in distilled water had strong antibacterial activity, whereas cell-free filtrate did not inhibit the growth of indicator strains.
ISSN:1230-0322