EFFECT OF DETERGENTS ON ACTIVITY OF ENDOLIPASES FROM RHIZOMUCOR MIEHEI AND YARROWIA LIPOLYTICA 2 - SHORT REPORT
 
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Pol. J. Food Nutr. Sci. 1998;48(3):526–532
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ABSTRACT
The aim of this work was to increase access and activity of lipases bounded to subcellular structures of Rhizomucor miehei and Yarrowia lipolytica. To obtain this goal biomass of fungi was treated with digitonin and cetyltrimethylammonium bromide (CTAB). The highest activity of endolipases was found for biomass incubated at 300C for 30 min and 0.2% digitonin for R. miehei and 0.2% CTAB for and Y. lipolytica . The lipolytic activity of permeabilized cells for both strains was about 2,5 tomes higher compared with native cells.
ISSN:1230-0322