ELECTROPHORETIC AND DSC STUDIES ON DIFFERENT WHEAT STARCHES WITH VARIOUS AMYLOSE CONTENTS
 
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Pol. J. Food Nutr. Sci. 2006;56(1):59–66
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ABSTRACT
Granule-bound starch synthase and the structural and thermodynamic properties of the isolated starches were compared using amylopectin (2.1% amylose content), normal (20.5-25.1% amylose content) and high amylose (≥39.5% amylose) wheat cultivars of Japanese, Italian and Russian selections. Amylopectin (2.1% amylose content), normal (20.5-25.1% amylose content) and high amylose (amylose rich containing ≥39.5% amylose) wheat cultivars were investigated using an electrophoretic technique to examine starch-granule bound synthase. High-sensitivity differential scanning microcalorimetry was used to examine the structural and thermodynamic properties of extracted wheat starches. Wx-B1 protein was not present in normal and even in high amylose cultivars except for the normal amylose cultivar Bilancia. An increase in amylose content in starches is generally accompanied by a decrease both in the melting enthalpy and the melting temperatures of crystalline lamella, whilst the polymorphous structure of starches remains invariable (A-type). The melting cooperative unit, the thickness and the surface free energy, enthalpy and entropy of crystalline lamella were also determined. Classification of wheat starches with different amylose contents is offered.
ISSN:1230-0322