Effect of High Methionine and Folic Acid Diet on The Level of Homocysteine and Lipid Profile in Experimental Rats
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Publication date: 2017-09-30
Pol. J. Food Nutr. Sci. 2017;67(3):219–224
The aim of this study was to reduce the level of homocysteine (Hcy) by high-methionine dietary supplementation of folic acid and to evaluate changes in lipid parameters in blood serum of experimental rats. The animals were randomly divided into seven groups (n=6, male), and were fed seven different diets for 52 days. The rodents were fed with the AIN-93G semi-synthetic diet (group I), supplemented with 32 g of methionine/kg diet (group II), whereas groups III-VII were fed with AIN-93G semi-synthetic diet supplemented with 32 g of methionine/kg diet and 2, 4, 8, 18 and 28 g of folic acid/kg diet, respectively. In rats serum, the content of homocysteine, total cholesterol level, HDL fraction and triacylglycerols were assessed enzymatically. In addition, the LDL+VLDL cholesterol content was calculated. The folic acid content in the diet was found to be highly negatively correlated with the homocysteine content (r=-0.95) and negatively correlated with the triacylglycerols content (r=-0.94) in the serum of rats. In the serum of rats which were fed the methionine diet with the highest-folic acid supplementation (28 mg folic acid/kg diet), the homocysteine and triglyceride contents were significantly lower, compared to the other types of experimental diets used.