FREE RADICALS AND THEIR ANALYSIS USING HIGH PERFORMANCE LIQUID CHROMATOGRAPHY
 
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Pol. J. Food Nutr. Sci. 2004;54(Special issue 2s):23–28
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ABSTRACT
The hydroperoxyl radical is a much stronger oxidant than superoxide anion-radical and was initially thought to be responsible for the toxic effects of oxygen and cell membrane damage [Liochev & Fridovich, 2001]. HO2· has a relatively long lifetime and so can diffuse to neighbouring structures. At the physiological pH of 7.4 about 1% of superoxide exists in its protonated form. A quantitative method for hydroperoxide determination has been developed by O'Gara et al. [Morisco et al., 2004], based on the oxidation of glutathione (GSH) by hydroperoxide and formation of oxidized glutathione (GSSG). This reaction is catalyzed by glutathione peroxidase. GSH and GSSG were separated on reversed phase column at pH 3 and their concentrations were estimated using electrochemical detection at a glassy carbon electrode. The method was applied for the analysis of picomolar levels of many hydroperoxides in human plasma.
ISSN:1230-0322