GEL FILTRATION OF CONDENSED TANNINS AND PHENOLIC ACIDS OF CANOLA HULLS ON SEPHADEX G-25 AND G-50
 
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Pol. J. Food Nutr. Sci. 2006;56(4):415–418
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ABSTRACT
Condensed tannins and phenolic acids were extracted from canola hulls into acetone-water (70:30; v/v) and fractionated using Sephadex G-25 and G-50 column chromatography with 50% (v/v) acetone as the mobile phase. Three fractions containing phenolic compounds were obtained from the extract using Sephadex G-25 and four using Sephadex G-50. The latter fractions eluted from the columns, packed with either gel, both gels were free of condensed tannins. The contents of total phenolics and condensed tannins were determined in the range of 52–280 mg/g, 0.198–1.153 A500/mg (vanillin method), and 0.333–1.330 A550 /mg (after n-butanol/HCl hydrolysis). The method described can be used as a step in the purification of condensed tannins of canola hulls before semi-preparative RP-18 HPLC column chromatography.
ISSN:1230-0322