LYTIC ENZYMES OF TRICHODERMA AND ITS UTILIZATION IN YEAST PROTOPLAST FORMATION.
 
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Pol. J. Food Nutr. Sci. 1999;49(2):245–252
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ABSTRACT
Trichoderma horzianum T-33 and Trichoderma hamatum c-1 are able to produce extracellular β-1,3- glucanases, chitanases, and cellulases in the shaked cultures, in the base medium enriched with mycelium (postcultural) or fodder yeast and supplemented with Avicel cellulose. The addition of fodder yeast (6%) and mycelium (6%) to the base medium induced more effective production of β-1,3-glucanases than the addition of lactose (22%). The production of chitanases and cellulases was the most successful in the medium enriched with mycelium. The enzymatic preparations from T. harzianum T-33 or T. hamatum C-1 (obtained after ethanol precipitation) were used for Yarrowia lipolytica ATCC 20461 protoplast formation (57.5% or 74.5% after 4h, respectively).
ISSN:1230-0322