NEW RAPID METHOD OF CACO-2 CELL DIFFERENTIATION
 
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Pol. J. Food Nutr. Sci. 2003;53(Special issue 1s):60–64
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ABSTRACT
The Caco-2 cell line is used by many investigators as a cellular intestinal model. The aim of this work was to improve the conventional long-term 21-day Caco-2 cell culture to obtain a simple, less expensive and more rapid culture system. In the new culture system, the cells were grown on polycarbonate filters in serum-reduced medium containing 2% iron-saturated calf serum supplemented with combination of growth factors and hormones. The modified culture conditions were designed to induce rapid differentiation of Caco-2 cells. Using this system, the differentiated enterocyte-like monolayer was established within 4-6 days and characterized for cell morphology, growth rate, glucose consumption rate, lactate production rate, the monolayer integrity and activity of alkaline phosphatase.
ISSN:1230-0322