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ORIGINAL ARTICLE
Pear Juice Clarification Using Polygalacturonase from Beauveria bassiana: Effects on Rheological, Antioxidant and Quality Properties
 
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Department of Biotechnology and Food Science, Faculty of Applied Sciences, Durban University of Technology, P O Box 1334, Durban, 4000, South Africa
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Department of Food Science and Technology, Chinhoyi University of Technology, P. Bag 7724, Chinhoyi, Zimbabwe
CORRESPONDING AUTHOR
Santhosh Pillai   

Department of Biotechnology and Food Science, Faculty of Applied Sciences, Durban University of Technology, P O Box 1334, Durban, 4000, South Africa., 4002, Durban, South Africa
Submission date: 2021-11-07
Final revision date: 2021-12-22
Acceptance date: 2022-01-11
Online publication date: 2022-02-03
Publication date: 2022-02-03
 
Pol. J. Food Nutr. Sci. 2022;72(1):57–67
 
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ABSTRACT
Although pear is one of the most preferred fruits globally due to its high nutrient content, its juice products have not received equal consumer acceptability due to their undesirable haziness and turbidity. However, enzymatic treatment of juice has been noted to improve the overall acceptability of juices better than traditional decantation, filtration, and heating methods, which are either expensive or detrimental to the product quality. In this study, pear juice was effectively clarified using partially purified polygalacturonase from an entomopathogenic fungal endophyte Beauveria bassiana SAN01. The optimization of the juice clarification process led to 137% improvement in clarification compared to control, under optimal conditions of 39.37 U/mL enzyme load, 36.87°C temperature and 2.75 h treatment time. Results also showed that the polygalacturonase treatment resulted in a significant decrease in viscosity and an increase in the pseudoplasticity of the pear juice as the rheological data of the juice was observed to fit adequately into the power law model (R2>0.9). Furthermore, the enzyme-assisted juice clarification was found to reduce browning index (-14.34%) and turbidity (-19.72%) while increasing the reducing sugar content (9.55%). Unlike some conventional juice treatments, the polygalacturonase treatment preserved the antioxidant potential and the total phenolic contents of the pear juice as no significant changes were observed after the treatment. This study thus demonstrates the efficacy of a fungal polygalacturonase in improving pear juice quality as well as the potential applicability of B. bassiana enzymes in the food industry.
FUNDING
This work was supported by the National Research Foundation of South Africa under grant numbers [UID 105447 and UID 114227].
 
REFERENCES (58)
1.
Abdullah, A.L., Sulaiman, N., Aroua, M., Noor, M.M.M. (2007). Response surface optimization of conditions for clarification of carambola fruit juice using a commercial enzyme. Journal of Food Engineering, 81(1), 65-71. https://doi.org/10.1016/j.jfoo....
 
2.
Adedeji, O.E., Ezekiel, O.O. (2020). Chemical composition and physicochemical properties of mango juice extracted using polygalacturonase produced by Aspergillus awamori CICC 2040 on pretreated orange peel. LWT – Food Science and Technology, 132, art. no. 109891. https://doi.org/10.1016/j.lwt.....
 
3.
Ahmed, A., Sohail, M. (2020). Characterization of pectinase from Geotrichum candidum AA15 and its potential application in orange juice clarification. Journal of King Saud University-Science, 32(1), 955-961. https://doi.org/10.1016/j.jksu....
 
4.
Al-juhaimi, F., Ghafoor, K., Özcan, M.M., Jahurul, M., Babiker, E.E., Jinap, S., Sahena, F., Sharifudin, M., Zaidul, I. (2018). Effect of various food processing and handling methods on preservation of natural antioxidants in fruits and vegetables. Journal of Food Science and Technology, 55(10), 3872-3880. https://doi.org/10.1007/s13197....
 
5.
Amin, F., Bhatti, H.N., Bilal, M., Asgher, M. (2017). Multiple parameter optimizations for enhanced biosynthesis of exo-polygalacturonase enzyme and its application in fruit juice clarification. International Journal of Food Engineering, 13(2), art. no. 20160256. https://doi.org/10.1515/ijfe-2....
 
6.
Amobonye, A., Bhagwat, P., Pandey, A., Singh, S., Pillai, S. (2020). Biotechnological potential of Beauveria bassiana as a source of novel biocatalysts and metabolites. Critical Reviews in Biotechnology, 40(7), 1019-1034. https://doi.org/10.1080/073885....
 
7.
Amobonye, A., Bhagwat, P., Singh, S., Pillai, S. (2021a). Beauveria bassiana xylanase: characterization and wastepaper deinking potential of a novel glycosyl hydrolase from an endophytic fungal entomopathogen. Journal of Fungi, 7(8), art. no. 668. https://doi.org/10.3390/jof708....
 
8.
Amobonye, A., Bhagwat, P., Singh, S., Pillai, S. (2021b). Enhanced xylanase and endoglucanase production from Beauveria bassiana SAN01, an entomopathogenic fungal endophyte. Fungal Biology, 125(1), 39-48. https://doi.org/10.1016/j.funb....
 
9.
Bailey, M.J., Biely, P., Poutanen, K. (1992). Interlaboratory testing of methods for assay of xylanase activity. Journal of Biotechnology, 23(3), 257-270. https://doi.org/10.1016/0168-1....
 
10.
Barman, S., Sit, N., Badwaik, L.S., Deka, S.C. (2015). Pectinase production by Aspergillus niger using banana (Musa balbisiana) peel as substrate and its effect on clarification of banana juice. Journal of Food Science and Technology, 52(6), 3579-3589. https://doi.org/10.1007/s13197....
 
11.
Benzie, I.F., Strain, J.J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP assay. Analytical Biochemistry, 239(1), 70-76. https://doi.org/10.1006/abio.1....
 
12.
Beveridge, T., Wrolstad, R.E. (1997). Haze and cloud in apple juices. Critical Reviews in Food Science and Nutrition, 37(1), 75-91. https://doi.org/10.1080/104083....
 
13.
Bora, S.J., Handique, J., Sit, N. (2017). Effect of ultrasound and enzymatic pre-treatment on yield and properties of banana juice. Ultrasonics Sonochemistry, 37, 445-451. https://doi.org/10.1016/j.ults....
 
14.
Cheng, S.H., Khoo, H., Ismail, A., Abdul-Hamid, A., Barakatun-Nisak, M. (2016). Influence of extraction solvents on Cosmos caudatus leaf antioxidant properties. Iranian Journal of Science and Technology, Transactions A: Science, 40(1), 51-58. https://doi.org/10.1007/s40995....
 
15.
Deshmukh, P.S., Manjunatha, S.S., Raju, P.S. (2015). Rheological behaviour of enzyme clarified sapota (Achras sapota L) juice at different concentration and temperatures. Journal of Food Science and Technology, 52(4), 1896-1910. https://doi.org/10.1007/s13197....
 
16.
Dıblan, S., Özkan, M. (2021). Effects of various clarification treatments on anthocyanins, color, phenolics and antioxidant activity of red grape juice. Food Chemistry, 352, art. no. 129321. https://doi.org/10.1016/j.food....
 
17.
Domingues, R.C., Junior, S.B., Silva, R.B., Madrona, G.S., Cardoso, V.L., Reis, M.H. (2011). Evaluation of enzymatic pretreatment of passion fruit juice. Chemical Engineering Transactions, 24, 517-522.
 
18.
Ern, K., Aron, H.A.H., Eng, C. (2016). Comparison of total phenolic contents (tpc) and antioxidant activities of fresh fruit juices, commercial 100% fruit juices and fruit drinks Sains Malaysiana, 45(9), 1319-1327.
 
19.
Gani, G., Naik, H., Jan, N., Bashir, O., Hussain, S.Z., Rather, A., Reshi, M., Amin, T. (2021). Physicochemical and antioxidant properties of pear juice prepared through pectinase enzyme-assisted extraction from William Bartlett variety. Journal of Food Measurement and Characterization, 15(1), 743-757. https://doi.org/10.1007/s11694....
 
20.
Gonzalez Viejo, C., Torrico, D.D., Dunshea, F.R., Fuentes, S. (2019). Emerging technologies based on artificial intelligence to assess the quality and consumer preference of beverages. Beverages, 5(4), art. no. 62. https://doi.org/10.3390/bevera....
 
21.
Gu, Q., Duan, G., Yu, X. (2019). Bioconversion of flavonoid glycosides from Hippophae rhamnoides leaves into flavonoid aglycones by Eurotium amstelodami. Microorganisms, 7(5), art. no. 122. https://doi.org/10.3390/microo....
 
22.
Islam, M.S., Patras, A., Pokharel, B., Wu, Y., Vergne, M.J., Shade, L., Xiao, H., Sasges, M. (2016). UV-C irradiation as an alternative disinfection technique: Study of its effect on polyphenols and antioxidant activity of apple juice. Innovative Food Science and Emerging Technologies, 34, 344-351. https://doi.org/10.1016/j.ifse....
 
23.
Jiang, G.H., Kim, Y.M., Nam, S.H., Yim, S.H., Eun, J.B. (2016). Enzymatic browning inhibition and antioxidant activity of pear juice from a new cultivar of asian pear (Pyrus pyrifolia Nakai cv. Sinhwa) with different concentrations of ascorbic acid. Food Science and Biotechnology, 25(1), 153-158. https://doi.org/10.1007/s10068....
 
24.
Kareem, S., Akpan, I., Popoola, T., Sanni, L. (2011). Activated charcoal – a potential material in glucoamylase recovery. Enzyme Research, 2011, art. no. 483943. https://doi.org/10.4061/2011/4....
 
25.
Karmakar, S., De, S. (2019). Pectin removal and clarification of juices. Chapter 5, In: Ch.M. Galanakis (Ed.), Separation of Functional Molecules in Food by Membrane Technology, Elsevier, Academic Press, pp. 155-194. https://doi.org/10.1016/B978-0....
 
26.
Koley, T.K., Walia, S., Nath, P., Awasthi, O., Kaur, C. (2011). Nutraceutical composition of Zizyphus mauritiana Lamk (Indian ber): effect of enzyme-assisted processing. International Journal of Food Sciences and Nutrition, 62(3), 276-279. https://doi.org/10.3109/096374....
 
27.
Kumar, K., Arumugam, N., Permaul, K., Singh, S. (2016). Thermostable enzymes and their industrial applications. Chapter 5, In: P. Shukla (Ed.), Microbial Biotechnology Interdisciplinary Approach, 1st ed., CRC Press, Boca Raton, USA, pp. 115-162. https://doi.org/10.1201/978131....
 
28.
Kyamuhangire, W., Myhre, H., Sørensen, H.T., Pehrson, R. (2002). Yield, characteristics and composition of banana juice extracted by the enzymatic and mechanical methods. Journal of the Science of Food and Agriculture, 82(4), 478-482. https://doi.org/10.1002/jsfa.1....
 
29.
Mandalari, G., Bennett, R.N., Kirby, A.R., Lo Curto, R.B., Bisignano, G., Waldron, K.W., Faulds, C.B. (2006). Enzymatic hydrolysis of flavonoids and pectic oligosaccharides from bergamot (Citrus bergamia Risso) peel. Journal of Agricultural and Food Chemistry, 54(21), 8307-8313. https://doi.org/10.1021/jf0615....
 
30.
Moon, K.M., Kwon, E.B., Lee, B., Kim, C.Y. (2020). Recent trends in controlling the enzymatic browning of fruit and vegetable products. Molecules, 25(12), art. no. 2754. https://doi.org/10.3390/molecu....
 
31.
Narnoliya, L.K., Jadaun, J.S., Chownk, M., Singh, S.P. (2020). Enzymatic systems for the development of juice clarification strategies. Chapter 18, In: S.P. Singh, A. Pandey, R.R. Singhania, Ch. Larroche, Z. Li (Eds.), Biomass, Biofuels, Biochemicals – Advances in Enzyme Catalysis and Technologies, Elsevier, pp. 397-412. https://doi.org/10.1016/B978-0....
 
32.
Nicoli, M.C., Elizalde, B.E., Pitotti, A., Lerici, C.R. (1991). Effect of sugars and maillard reaction products on polyphenol oxidase and peroxidase activity in food. Journal of Food Biochemistry, 15(3), 169-184. https://doi.org/10.1111/j.1745....
 
33.
Ogando, F.I.B., de Aguiar, C.L., Viotto, J.V.N., Heredia, F.J., Hernanz, D. (2019). Removal of phenolic, turbidity and color in sugarcane juice by electrocoagulation as a sulfur-free process. Food Research International, 122, 643-652. https://doi.org/10.1016/j.food....
 
34.
Ordóñez‐Santos, L.E., Martínez‐Girón, J. (2020). Thermal degradation kinetics of carotenoids, vitamin C and provitamin A in tree tomato juice. International Journal of Food Science and Technology, 55(1), 201-210. https://doi.org/10.1111/ijfs.1....
 
35.
Petruzzi, L., Campaniello, D., Speranza, B., Corbo, M.R., Sinigaglia, M., Bevilacqua, A. (2017). Thermal treatments for fruit and vegetable juices and beverages: A literature overview. Comprehensive Reviews in Food Science and Food Safety, 16(4), 668-691. https://doi.org/10.1111/1541-4....
 
36.
Pradhan, D., Abdullah, S., Pradhan, R.C. (2020). Optimization of pectinase assisted extraction of chironji (Buchanania lanzan) fruit juice using response surface methodology and artificial neural network. International Journal of Fruit Science, 20(Suppl. 2), S318-S336. https://doi.org/10.1080/155383....
 
37.
Ramadan, M.F. (2019). Enzymes in fruit juice processing. Chapter 4, In: M. Kuddus (Ed.), Enzymes in Food Biotechnology - Production, Applications, and Future Prospects, Elsevier, Academic Press, pp. 45-59. https://doi.org/10.1016/B978-0....
 
38.
Salta, J., Martins, A., Santos, R.G., Neng, N.R., Nogueira, J.M., Justino, J., Rauter, A.P. (2010). Phenolic composition and antioxidant activity of Rocha pear and other pear cultivars–a comparative study. Journal of Functional Foods, 2(2), 153-157. https://doi.org/10.1016/j.jff.....
 
39.
Ribas-Agustí, A., Martín-Belloso, O., Soliva-Fortuny, R., Elez-Martínez, P. (2018). Food processing strategies to enhance phenolic compounds bioaccessibility and bioavailability in plant-based foods. Critical Reviews in Food Science and Nutrition, 58(15), 2531-2548. https://doi.org/10.1080/104083....
 
40.
Romero-González, J., Ah-Hen, K.S., Lemus-Mondaca, R., Muñoz-Fariña, O. (2020). Total phenolics, anthocyanin profile and antioxidant activity of maqui, Aristotelia chilensis (Mol.) Stuntz, berries extract in freeze-dried polysaccharides microcapsules. Food Chemistry, 313, art. no. 126115. https://doi.org/10.1016/j.food....
 
41.
Salehi, F. (2020). Physicochemical characteristics and rheological behaviour of some fruit juices and their concentrates. Journal of Food Measurement and Characterization, 14, 2472–2488. https://doi.org/10.1007/s11694....
 
42.
Sanchez, C., Blanco, D., Oria, R., Sánchez‐Gimeno, A.C. (2009). White guava fruit and purees: textural and rheological properties and effect of the temperature. Journal of Texture Studies, 40(3), 334-345. https://doi.org/10.1111/j.1745....
 
43.
Sandri, I.G., Silveira, M.M.d. (2018). Production and application of pectinases from Aspergillus niger obtained in solid state cultivation. Beverages, 4(3), art. no. 48. https://doi.org/10.3390/bevera....
 
44.
Santana, M.L., Bispo, J.A.C., de Sena, A.R., Teshima, E., de Brito, A.R., Costa, F.S., Franco, M., de Assis, S.A. (2021). Clarification of tangerine juice using cellulases from Pseudoyma sp. Journal of Food Science and Technology-Mysore, 58(1), 44-51. https://doi.org/10.1007/s13197....
 
45.
Saxena, D., Sabikhi, L., Chakraborty, S.K., Singh, D. (2014). Process optimization for enzyme aided clarification of watermelon juice. Journal of Food Science and Technology, 51(10), 2490-2498. https://doi.org/10.1007/s13197....
 
46.
Schieber, A., Keller, P., Carle, R. (2001). Determination of phenolic acids and flavonoids of apple and pear by high-performance liquid chromatography. Journal of Chromatography A, 910(2), 265-273. https://doi.org/10.1016/S0021-....
 
47.
Sethi, S., Joshi, A., Arora, B., Bhowmik, A., Sharma, R., Kumar, P. (2020). Significance of FRAP, DPPH, and CUPRAC assays for antioxidant activity determination in apple fruit extracts. European Food Research and Technology, 246(3), 591-598. https://doi.org/10.1007/s00217....
 
48.
Shankar, S., Laxman, R.S. (2015). Biophysicochemical characterization of an alkaline protease from Beauveria sp. MTCC 5184 with multiple applications. Applied Biochemistry and Biotechnology, 175(1), 589-602. https://doi.org/10.1007/s12010....
 
49.
Sharma, H.P., Patel, H., Sugandha, (2017). Enzymatic added extraction and clarification of fruit juices – a review. Critical Reviews in Food Science and Nutrition, 57(6), 1215-1227. https://doi.org/10.1080/104083....
 
50.
Singh, V., Jawandha, S., Gill, P. (2021). Effect of exogenous putrescine treatment on internal browning and colour retention of pear fruit. Journal of Food Measurement and Characterization, 15, 905–913. https://doi.org/10.1007/s11694....
 
51.
Sorrivas, V., Genovese, D., Lozano, J. (2006). Effect of pectinolytic and amylolytic enzymes on apple juice turbidity. Journal of Food Processing and Preservation, 30(2), 118-133. https://doi.org/10.1111/j.1745....
 
52.
Tavares, D.T., Alcantara, M.R., Tadini, C.C., Telis-Romero, J. (2007). Rheological properties of frozen concentrated orange juice (FCOJ) as a function of concentration and subzero temperatures. International Journal of Food Properties, 10(4), 829-839. https://doi.org/10.1080/109429....
 
53.
Thaochan, N., Ngampongsai, A., Prabhakar, C.S., Hu, Q. (2021). Beauveria bassiana PSUB01 simultaneously displays biocontrol activity against Lipaphis erysimi (Kalt.)(Hemiptera: Aphididae) and promotes plant growth in Chinese kale under hydroponic growing conditions. Biocontrol Science and Technology, 31(10), 997-1015. https://doi.org/10.1080/095831....
 
54.
Uçan, F., Ağçam, E., Akyildiz, A. (2016). Bioactive compounds and quality parameters of natural cloudy lemon juices. Journal of Food Science and Technology, 53(3), 1465-1474. https://doi.org/10.1007/s13197....
 
55.
Wang, J., Vanga, S.K., Raghavan, V. (2019). High-intensity ultrasound processing of kiwifruit juice: Effects on the ascorbic acid, total phenolics, flavonoids and antioxidant capacity. LWT – Food Science and Technology, 107, 299-307. https://doi.org/10.1016/j.lwt.....
 
56.
Xie, D., Zhong, H.-Y., Mo, J., Li, Z.-H., Cui, T., Yi, C.-P. (2007). Nutritional and medicinal quality of pear juice: Next hotspot?. Food, 1(1), 41-48.
 
57.
Yang, S.Q., Dai, X.Y., Wei, X.Y., Zhu, Q., Zhou, T. (2019). Co‐immobilization of pectinase and glucoamylase onto sodium aliginate/graphene oxide composite beads and its application in the preparation of pumpkin–hawthorn juice. Journal of Food Biochemistry, 43(3), art. no. e12741. https://doi.org/10.1111/jfbc.1....
 
58.
Zhao, D., Wang, Y., Na, J., Ping, W., Ge, J. (2019). The response surface optimization of β-mannanase produced by Lactobacillus casei HDS-01 and its potential in juice clarification. Preparative Biochemistry and Biotechnology, 49(2), 202-207. https://doi.org/10.1080/108260....
 
 
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