REPRODUCIBILITY OF TWO-DIMENSIONAL ELECTROPHORESIS GEL IMAGES OF PEA (PISUM SATIVUM L.) SEED PROTEINS EVALUATED USING SCATTER PLOTS
 
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Pol. J. Food Nutr. Sci. 2009;59(2):141–144
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ABSTRACT
The aim of this study was to evaluate plots showing a linear correlation between the parameters characterising protein content in the spots obtained using two-dimensional electrophoresis (scatter plots). The correlation coefficient of normalized spot volume may be used to determine regions with increased reproducibility. In our experiment, conducted using pea seed proteins, the correlation coefficient reached 0.980 in such regions, compared with 0.917 noted for the entire gel surface. The correlation coefficient calculated based on scatter plots may serve as a tool for the preliminary selection of regions characteristic of a given proteome.
ISSN:1230-0322