RP-HPLC-DAD FINGER PRINT ANALYSIS OF PHENOLIC EXTRACTS FROM TRANSGENIC FLAX

Two flax lines overexpressing genes encoding three essential enzymes of the flavonoid synthesis pathway: chalcone synthase (CHS), chalcone isomerase (CHI), and dihydroflavonol reductase (DFR), were generated. Phenolic compounds were extracted from the flaxseed lines and the control sample (Linola) using 80% methanol. Then the complex of phenolic compounds present in the crude extract was hydrolysed at basic conditions. The crude and hydrolysed extracts were analysed using an RP-HPLC-DAD method. The chromatograms of the crude extracts of the control sample and transgenic lines were the same. The finger print analysis of the hydrolysed extracts shows the influence of the expression of genes encoding three above mentioned enzymes on the proportion between secoisolariciresinol diglucoside (SDG) and phenolic acids derivatives forming the phenolic complex in flaxseed.

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eISSN:	2083-6007
ISSN:	1230-0322

W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.