SYNTHESIS, PROPERTIES, AND APPLICATION OF LIPASE FROM CANDIDA ANTARCTICA FOR HIGH YIELD MONOACYLGLYCEROL BIOSYNTHESIS.
 
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Pol. J. Food Nutr. Sci. 2003;53(4):3–8
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ABSTRACT
Synthesis of extracellular lipases by Candida antarctica was induced by adding fat or oil to the culture medium. The highest lipase activity (9.1 U) was obtained after 72 h of cultivation of yeast in medium supplemented with 2% (v/v) 1,2,3-trioleoyl-sn-glycerol. On the basis of SDS-PAGE analysis, Candida antarctica synthesized two forms of lipase having molecular weight of 36 and 52 kDa. Lipase from Candida antarctica showed the highest activity at pH 8.0 and temperature of 35oC and was stable in this range of temperature and pH. Activity of Candida antarctica lipase was higher on substrates having short-chain and medium-chain fatty acids than on these containing long-chain fatty acids. Using Candida antarctica lipase immobilized on Celite 545 in the synthesis of monoacylglycerols, 100% of conversion was obtained after esterification of oleic acid with isopropylidene glycerol. After hydrolysis of protected monoacylglycerols with trifluoroacetic acid, pure monoacylglycerols were obtained.
ISSN:1230-0322