The study was aimed at determining the effect of various doses of tocopherol acetate on the oxidative stress induced by physical exercise. The experiment was conducted on growing Wistar male rats. The oxidative stress was induced by running on a treadmill with tape movement velocity of 20 m/min for 15 min for the period of 10 days. After that period, samples of rats serum were determined for levels of α-tocopherol and lipid peroxides as well as for antioxidative potential and α-tocopherol content of liver. The study demonstrated that physical exercise was leading to enhanced release of tocopherol from liver to blood, depending on the applied dose of the vitamin. The serum level of lipid peroxides was lower in the animals subjected to the training and inversely proportional to the dose of tocopherol. In the animals not subjected to exercise, the oxidative stress was observed to be enhanced at the highest dose of the vitamin.