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3/2017 vol. 67
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Effect of High Methionine and Folic Acid Diet on The Level of Homocysteine and Lipid Profile in Experimental Rats
Renata Bieżanowska-Kopeć
,
 
Teresa Leszczyńska
 
 
 
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Publication date: 2017-09-30
 
 
Pol. J. Food Nutr. Sci. 2017;67(3):219-224
DOI: https://doi.org/10.1515/pjfns-2016-0027
 
 
Article (PDF)
References (38) Citations (1)
 
KEYWORDS
methionine
folic acid
homocysteine
lipid profile
rats
 
ABSTRACT
The aim of this study was to reduce the level of homocysteine (Hcy) by high-methionine dietary supplementation of folic acid and to evaluate changes in lipid parameters in blood serum of experimental rats. The animals were randomly divided into seven groups (n=6, male), and were fed seven different diets for 52 days. The rodents were fed with the AIN-93G semi-synthetic diet (group I), supplemented with 32 g of methionine/kg diet (group II), whereas groups III-VII were fed with AIN-93G semi-synthetic diet supplemented with 32 g of methionine/kg diet and 2, 4, 8, 18 and 28 g of folic acid/kg diet, respectively. In rats serum, the content of homocysteine, total cholesterol level, HDL fraction and triacylglycerols were assessed enzymatically. In addition, the LDL+VLDL cholesterol content was calculated. The folic acid content in the diet was found to be highly negatively correlated with the homocysteine content (r=-0.95) and negatively correlated with the triacylglycerols content (r=-0.94) in the serum of rats. In the serum of rats which were fed the methionine diet with the highest-folic acid supplementation (28 mg folic acid/kg diet), the homocysteine and triglyceride contents were significantly lower, compared to the other types of experimental diets used.
 
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CITATIONS (1):
1.
Hepatotoxicity and biochemical evaluation of a novel breakfast food produced from the blend of fonio (Digitaria iburua Stapf) and pigeon pea (Cajanus cajan (L.) Millspaugh) in albino rats
Grace Babarinde, Akos Ebun, Peter Adegbola
Bulletin of the National Research Centre
 
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