APPLICATION OF MULTIPLEX PCR IN ROUTINE MICROBIOLOGICAL DIAGNOSTICS OF LISTERIA MONOCYTOGENES AND LISTERIA SP. STRAINS IN A MEAT-PROCESSING PLANT.

A multiplex PCR assay designed by Bansal [1996] was applied in a routine screening test of a meat-processing environment aimed at detection of Listeria monocytogenes and species of the genus Listeria. Listeria spp. strains yielded a single 938-bp product indicating presence of 16S rRNA conservative sequence typical of the genus, whereas L. monocytogenes strains yielded not only the 938-bp product but also a 750-bp product - as a result of amplification within region of the listeriolysin (hly A) gene. The assay was used to verify identification of 50 colonies performed using classical tests, including catalase and hemolytic activity, motility and API®LISTERIA (bioMerieux) biochemical tests. Among isolates, 4 strains (8%) were characterised by contradictory results of biochemical and genetic tests. The possibility of simultaneous identification and differentiation of potentially pathogenic L. monocytogense strains from other Listeria spp. during one reaction performed was proved.

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PREDICTION LISTERIA MONOCYTOGENES GROWTH IN MILK – COMPARISON WITH PATHOGEN MODELING PROGRAM 7.0

EVALUATION OF EFFICACY OF TESTS RECOMMENDED BY A PRPN EN ISO 11290-1:1999 STANDARD FOR IDENTIFICATION OF LISTERIA SPP. AND LISTERIA MONOCYTOGENES ISOLATED FROM MEAT AND MEAT-PROCESSING ENVIRONMENT.

COMPARATIVE STUDIES ON IDENTIFICATION OF LISTERIA MONOCYTOGENES STRAINS PERFORMED USING A COMMERCIAL API®LISTERIA KIT AND PCR TECHNIQUE

PREVALENCE OF LISTERIA SPP. IN MEAT AND RAW SAUSAGES.

THE ISOLATION AND DETECTION OF LISTERIA FROM ARTIFICIALLY CONTAMINATED FOOD BY USE OF IMMUNOMAGNETIC SEPARATION AND CULTIVATION PROCEDURES.

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eISSN:	2083-6007
ISSN:	1230-0322

W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.