CHROMATOGRAPHIC SEPARATION OF TANNIN FRACTIONS FROM A BEARBERRY-LEAF ( ARCTOSTAPHYLOS UVA-URSI  L. SPRENGEL) EXTRACT BY SE-HPLC – A SHORT REPORT

Phenolic compounds from the leaves of the bearberry plant (Arctostaphylos uva-ursi L. Sprengel) were extracted into 95% (v/v) ethanol. Tannin fractions were obtained by Sephadex LH-20 column chromatography using 50% (v/v) acetone as the mobile phases. The tannin fraction was then further characterised by size exclusion high-performance liquid chromatography (SE-HPLC) on a TSK G2000SW_XL column with a mobile phase comprising 45% acetonitrile and 0.1% (v/v) TFA. The chromatography revealed the presence of additional phenolic compounds in the tannin fractions which are not proanthocyanidins (i.e. condensed tannins).

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EXTRACTION AND CHROMATOGRAPHIC SEPARATION OF TANNIN FRACTIONS FROM TANNIN-RICH PLANT MATERIAL

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eISSN:	2083-6007
ISSN:	1230-0322

W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.