CHROMATOGRAPHIC SEPARATION OF TANNIN FRACTIONS FROM A BEARBERRY-LEAF ( ARCTOSTAPHYLOS UVA-URSI L. SPRENGEL) EXTRACT BY SE-HPLC – A SHORT REPORT

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CHROMATOGRAPHIC SEPARATION OF TANNIN FRACTIONS FROM A BEARBERRY-LEAF (ARCTOSTAPHYLOS UVA-URSI L. SPRENGEL) EXTRACT BY SE-HPLC – A SHORT REPORT

Ronald B. Pegg

,

Anna Rybarczyk

,

Ryszard Amarowicz

Publication date: 2008-12-31

Pol. J. Food Nutr. Sci. 2008;58(4):485-490

KEYWORDS

ABSTRACT

Phenolic compounds from the leaves of the bearberry plant (Arctostaphylos uva-ursi L. Sprengel) were extracted into 95% (v/v) ethanol. Tannin fractions were obtained by Sephadex LH-20 column chromatography using 50% (v/v) acetone as the mobile phases. The tannin fraction was then further characterised by size exclusion high-performance liquid chromatography (SE-HPLC) on a TSK G2000SW_XL column with a mobile phase comprising 45% acetonitrile and 0.1% (v/v) TFA. The chromatography revealed the presence of additional phenolic compounds in the tannin fractions which are not proanthocyanidins (i.e. condensed tannins).

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