IMMUNOGENIC POTENTIAL OF ANTIGENS ISOLATED FROM TRYPSIN PEA PROTEIN HYDROLYSATES

The study was undertaken to examine the immunogenic potential of pea protein of Polish cultivar Maraton and its trypsin hydrolysates differing in a degree of hydrolysis. The physicochemical characteristics of a pea protein extract and its hydrolysates, DH 2.0 and 5.0, was conducted by means of SDS-PAGE electrophoresis, chromatofocusing, affinity chromatography, and sequential analysis. The immunogenic properties of pea protein and its trypsin hydrolysates, DH 2.0 and 5.0, were investigated by direct and competitive ELISA methods. The results confirm that the protein extract is a stronger immunogene than the hydrolysates, while hydrolysate with DH 2.0 was a stronger immunogene than that with DH 5.0. The dominant antigen isolated from the pea protein extract and both trypsin hydrolysates had a molecular weight of about 20 kDa and was in glycoprotein fraction. The N-terminal sequence of this antigen was determined to be: Thr-Glu-Thr-Thr-Ser-Phe-Leu-Ile-Thr-Lys. Its precursor is probably pea lectin.

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eISSN:	2083-6007
ISSN:	1230-0322

W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.