EFFECT OF DETERGENTS ON ACTIVITY OF ENDOLIPASES FROM  RHIZOMUCOR MIEHEI  AND  YARROWIA LIPOLYTICA  2 - SHORT REPORT

The aim of this work was to increase access and activity of lipases bounded to subcellular structures of Rhizomucor miehei and Yarrowia lipolytica. To obtain this goal biomass of fungi was treated with digitonin and cetyltrimethylammonium bromide (CTAB). The highest activity of endolipases was found for biomass incubated at 30⁰C for 30 min and 0.2% digitonin for R. miehei and 0.2% CTAB for and Y. lipolytica . The lipolytic activity of permeabilized cells for both strains was about 2,5 tomes higher compared with native cells.

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eISSN:	2083-6007
ISSN:	1230-0322

W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.