IMMOBILISATION OF THE PROTEOLYTIC ENZYME SYSTEM (CHYMOTRYPSIN-EXOPEPTIDASE) IN HIGH-MOLECULAR POLYSACCHARIDES.

Three kinds of high-molecular polysaccharides alginate, chitosan and carrageenan were tested as carriers for the immobilisation of proteolytic enzymes. The catalytic system consisted of the endopeptidase - chymotrypsin and exopeptidases: carboxypeptidase A and leucine aminopeptidase. Subsequently, this immobilised system was used for making of enzymatic hydrolysates of casein. In the case of periodic method of casein hydrolysis the best results (71.9% of tyrosine and tryptophan released) were achieved after 72 h, at substrate concentration 0.5% and with alginate as a carrier. In the semi-continuous method chitosan appeared to be the best carrier and after 72 h the degree of hydrolysis was 48.6% (52.7% of tyrosine and tryptophan released) at the concentration of casein 2%.

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eISSN:	2083-6007
ISSN:	1230-0322

W latach 2022-2024 kwartalnik naukowy Polish Journal of Food and Nutrition Sciences realizuje projekt nr RCN/SP/0520/2021/1 finansowany ze środków budżetu państwa – Ministerstwo Edukacji i Nauki w ramach programu „Rozwój czasopism naukowych”. Dofinansowanie wynosi 120 000 zł. W ramach projektu podejmowane są działania zmierzające do podniesienia poziomu praktyk wydawniczych i edytorskich, zwiększenia wpływu czasopism na rozwój nauki oraz utrzymania się czasopism w międzynarodowym obiegu naukowym.

In the years 2022-2024, the scientific quarterly Polish Journal of Food and Nutrition Sciences accomplishes a project no. RCN/SP/0520/2021/1 financed from the state budged – Ministry of Education and Science Republic of Poland in the framework of a program “Development of scientific journals”. The financing amounts to 120,000 PLN. The program aims at improving the level of publishing and editing practices, increasing the impact of scientific journals on science development, and extending the international range of scientific journals.