The aim of performed researches was an evaluation of the influence of UV radiation usage on the population of microorganisms settling the aloe pulp. The research materials were leaves of a three-year aloe. The aloe pulp in two forms (with skin and without skin) was prepared according to the patent application P346068. Part of prepared pulp was subjected to analyses before being exposed to the UV radiation process, while the other part was divided and treated with UV radiation with a range of 240–280 nm for 10 and 20 min. Microbiological analyses were performed on raw pulp before irradiation, in pulp exposed to irradiation (1 day after irradiation) and 30 days of storage. In the researched material the total counts of mesophile bacteria, total counts of Staphylococcus aureus and total counts of yeasts and fungi were determined. The study demonstrated an increase in the numbers of coagulase positive and coagulase negative Staphylococcus aureus after 30 days of storage of a preserved homogenate at ±4ºC. After a 30-day storage period of preserved homogenate with skin and without skin there was observed an increase of mesophilic microflora population from values of 3.11 and 2.47 log cfu/g to 7.48 log cfu/g in both cases. The number of yeasts living in the aloe pulp with skin and without skin, after being subjected to the process of preservation and storage for the period of 30 days, increased from about 1.5 to 5 logarithmic cycles. During the 30-day storage period of UV preserved aloes pulp it was observed that there was a significant overgrowth of researched homogenate by the population of shred fungi.